Neuroprotective and antidiabetic lanostane-type triterpenoids from the fruiting bodies of Ganoderma theaecolum  

作　　者：Jiaocen Guo Li Yang Luting Dai Qingyun Ma Jiaoyang Yan Qingyi Xie Yougen Wu Haofu Dai Youxing Zhao 

机构地区：[1]School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication),Hainan University,Sanya 572025,China [2]Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province&National Key Laboratory for Tropical Crop Breeding,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China

出　　处：《Chinese Journal of Natural Medicines》2025年第2期245-256,共12页中国天然药物(英文版)

基　　金：supported by the National Natural Science Foundation of China (No.81973568);the Earmarked Fund for China Agriculture Research System (No.CARS-21);the Special Financial Project of the Ministry of Agriculture and Rural Areas (No.NFZX2024)。

摘　　要：Eight previously undescribed lanostane triterpenoids,including five nortriterpenoids with 26carbons,ganothenoids A-E(1-5),and three lanostanoids,ganothenoids F-H(6-8),along with 24 known ones(9-32),were isolated from the fruiting bodies of Ganodrma theaecolum.The structures of the novel compounds were elucidated using comprehensive spectroscopic methods,including electronic circular dichroism(ECD)and nuclear magnetic resonance(NMR)calculations.Compounds 1-32 were assessed for their neuroprotective effects against H_(2)O_(2)-induced damage in human neuroblastoma SH-SY5Y cells,as well as their inhibitory activities against protein tyrosine phosphatase 1B(PTP1B)andα-glucosidase.Compound 4demonstrated the most potent neuroprotective activity against H_(2)O_(2)-induced oxidative stress by suppressing G_0/G_1 phase cell cycle arrest,reducing reactive oxygen species(ROS)levels,and inhibiting cell apoptosis through modulation of B-cell lymphoma 2 protein(Bcl-2)and Bcl-2 associated X-protein(Bax)protein expression.Compounds 26,12,and 28 exhibited PTP1B inhibitory activities with IC_(50)values ranging from 13.92 to 56.94μmol·L~(-1),while compound12 alone displayed significant inhibitory effects onα-glucosidase with an IC_(50)value of 43.56μmol·L~(-1).Additionally,enzyme kinetic analyses and molecular docking simulations were conducted for compounds 26 and 12 with PTP1B andα-glucosidase,respectively.

关 键 词：Ganoderma theaecolum Lanostane nortriterpenoids Neuroprotective effects PTP1B Α-GLUCOSIDASE 

分 类 号：R73[医药卫生—肿瘤]