大黄煎剂调节大鼠肠道微生态干预轻微型肝性脑病  

Rhubarb Decoction Alleviates Minimal Hepatic Encephalopathy via Regulating Intestinal Microecology of Rats

作　　者：王萌[1] 姚春[2] 杜沅沁 黄良江 付蕾[2] 蔡颖莹 庞娣 WANG Meng;YAO Chun;DU Yuan-qin;HUANG Liang-jiang;FU Lei;CAI Ying-ying;PANG Di(Department of Spleen and Stomach Diseases,The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023;Engineering Research Center of Innovative Drugs for Traditional Chinese Medicine and Zhuang&Yao Medicine,Guangxi University of Chinese Medicine,Nanning 530001;Graduate school,Guangxi University of Chinese Medicine,Nanning 530001;Department of Hepatology,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023;Internal Medicine Department,Guangxi University of Chinese Medicine Ren'ai Branch,Nanning 530001)

机构地区：[1]广西中医药大学第一附属医院脾胃病科,南宁530023 [2]广西中医药大学中药壮瑶药创新药物教育部工程研究中心,南宁530001 [3]广西中医药大学研究生院,南宁530001 [4]广西中医药大学第一附属医院肝病科,南宁530023 [5]广西中医药大学第一附属医院仁爱分院内科门诊,南宁530001

出　　处：《中国中西医结合杂志》2025年第2期205-212,共8页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广西自然科学基金重点项目(No.桂科AB22035076);广西自然科学基金青年基金(No.2020GXNSFBA297033);国家自然科学基金地区基金(No.82260899);广西中医壮瑶医治疗优势病种研究项目(No.GZBZ22-3);广西中医药大学“桂派中医药传承创新团队”资助项目(No.2022A001);广西研究生教育创新项目(No.YCSW2022343)。

摘　　要：目的 通过16SrDNA测序技术分析大黄煎剂干预轻微型肝性脑病大鼠后肠道菌群变化情况,探讨中药大黄煎剂的作用靶点。方法 将45只大鼠随机分为对照组(NC组)、轻微型肝性脑病模型组(MHE组)、大黄煎剂干预组(MHEY组:醋制大黄30 g乌梅30 g),每组15只。采用腹腔注射四氯化碳(CCl4)和硫代乙酰胺(TAA)诱导MHE大鼠模型,造模成功后各组选取10只进行对比分析。采用水迷宫实验评价各组大鼠干预前后学习记忆能力,分析生化指标,16SrDNA测序技术分析各组大鼠肠道菌群的数量、结构和丰度变化。结果 与NC组比较,MHE组寻台时间延长,丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)、总胆汁酸(TBA)、血氨均明显升高,白蛋白(ALB)下降(P<0.05);与MHE组比较,MHEY组水迷宫寻台时间缩短,AST、ALT、TBIL、TBA及血氨减少,ALB升高(P<0.05)。16SrDNA研究发现:与NC组比较,MHE组、MHEY组厚壁菌门、蓝藻细菌门均下调,拟杆菌门、放线菌门均上调(P<0.05);MHE组有害菌增加;MHEY组有益菌增加明显,并且放线菌门有下调趋势,乳杆菌属为优势菌群。MHEY组微生物标志物为:梭菌属、梭菌目、瘤胃球菌科、疣微菌科、毛螺菌科等。结论 大黄煎剂可下调MHE大鼠肠道有害菌群的菌落数量,通过中医学“通腑开窍法”酸化肠道,重建肠道菌群稳态,有利于MHE向愈。Objective To analyze the changes of intestinal flora of rats with minimal hepatic encephalopathy(MHE)after the treatment of Rhubarb Decoction using 16SrDNA sequencing technology, and to explore the changes of intestinal flora of MHE, as well as the target of action of Rhubarb Decoction.Methods Totally 45 rats were randomly divided into the normal control group(NC), the MHE model group(MHE), and the treatment group(MHEY) for the 30 g of Mume fructus and 30 g of vinegar-made Rhubarb,15 in each group. Rat in the MHE and HE groups were induced by intraperitoneal injection of tetrachloromethane(CCl4)and thioacetamide(TAA). After successful modeling, 10 rats in each group were selected for comparative analysis. The water maze experiment was used to evaluate the learning and memory abilities of rats in each group before and after treatment, and biochemical indices were analyzed. 16SrDNA sequencing technology was used to analyze the changes in the number, structure, and abundance of the intestinal flora of rats in each group.Results Compared with the NC group,the water maze test search time was prolonged, the levels of aspartate transaminase(AST), alanine aminotransferase(ALT), total bilirubin(TBIL), total bile acid(TBA), and blood ammonia increased,ALB decreased(P<0.05)in the MHE group. Compared with the MHE group, the water maze test search time was shortened, the levels of AST, ALT, TBIL, TBA, and blood ammonia decreased, ALB increased(P<0.05)in the MHEY group. 16SrDNA technology revealed that, compared with group NC, Firmicutes and Cyanobacteria were down-regulated, Bacteroidetes and Actinobacteria were up-regulated in MHE group and MHEY group. While the good bacteria proliferated in group MHEY, the detrimental bacteria rose dramatically in group MHE. Additionally, actinomyces in group MHEY had a declining tendency, and lactobacillus emerged as the dominating species following treatment of Rhubarb Decoction. The Clostridium genus, Clostridia order, Ruminococcaceae, Verrucomycetes family, Trichospiridae family, etc. w

关 键 词：16SrDNA测序 大黄煎剂 轻微型肝性脑病 肠道菌群 中药复方 中西医结合 

分 类 号：R28[医药卫生—中药学]