蒸制三七化学成分研究  

Study on chemical constituents of steamed Panax notoginseng

作　　者：程明赛 韩竹箴[1] 姜淞凡 张亦云 王振中 王峥涛[1] 杨颖博[1,2,3] 肖伟[1,2,3] CHENG Mingsai;HAN Zhuzhen;JIANG Songfan;ZHANG Yiyun;WANG Zhenzhong;WANG Zhengtao;YANG Yingbo;XIAO Wei(Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;National Key Laboratory of Pharmaceutical Process Control and Intelligent Manufacture Technologies for Chinese Medicine,Lianyungang 222001,Jiangsu,China;Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,Jiangsu,China)

机构地区：[1]上海中医药大学中药研究所,上海201203 [2]中药制药过程控制与智能制造技术全国重点实验室,江苏连云港222001 [3]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《上海中医药大学学报》2025年第1期20-28,共9页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(82130115,81920108033);江苏工业和信息化厅项目。

摘　　要：目的:研究蒸制三七中的化学成分。方法:蒸制三七70%乙醇提取物采用MCITMGEL填料-十八烷基硅烷键合硅胶填料(ODS)柱色谱方法,结合半制备型高效液相色谱进行纯化分离,根据波谱数据分析与文献比对进行化合物的结构鉴定。对所有化合物进行二氢乳清酸脱氢酶(DHODH)抑制活性筛选。结果:从蒸制三七中分离得到16个化合物,分别鉴定为(3β,6α,12β,20E)-3,12-dihydroxydammara-20(22),24-dien-6-yl-3-O-β-D-xylopyranosyl-β-Dglucopyranoside(1)、20-(R)-人参皂苷Rh1(2)、人参皂苷Rk1(3)、人参皂苷Rg5(4)、人参皂苷Rk3(5)、三七皂苷R2(6)、20-(R)-三七皂苷R2(7)、20-(S)-人参皂苷Rg2(8)、20-(R)-人参皂苷Rg2(9)、20-(S)-人参皂苷Rh1(10)、三七皂苷B1(11)、三七皂苷ST13(12)、3β,12β,25-trihydroxydammar-(E)-20(22)-ene-6-O-β-D-xylopyranosyl-(1→2)-β-Dglucopyranoside(13)、三七皂苷T5(14)、20-(S)-人参皂苷Rg3(15)、20-(R)-人参皂苷Rg3(16)。结论:化合物1为首次从蒸制三七中分离得到,对该化合物的核磁数据NMR进行了补充;化合物7对DHODH活性的抑制率为58.74%。Objective:To study the chemical constituents of steamed Panax notoginseng.Methods:The 70%ethanol extract of steamed Panax notoginseng was purified and separated by MCITMgel-octadecylsilane chemically bonded silica gel filler(ODS)column chromatography combined with semi-preparative high performance liquid chromatography,and structures of the compounds were identified by spectral data analysis and literature comparison.All compounds were screened for dihydroorotate dehydrogenase(DHODH)inhibitory activity.Results:16 compounds were isolated from steamed Panax notoginseng,and identified as(3β,6α,12β,20E)-3,12-dihydroxydammara-20(22),24-dien-6-yl-3-O-β-D-xylopyranosyl-β-D-glucopyranoside(1),20-(R)-ginsenoside Rh1(2),ginsenoside Rk1(3),ginsenoside Rg5(4),ginsenoside Rk3(5),notoginsenoside R2(6),20-(R)-notoginsenoside R2(7),20-(S)-ginsenoside Rg2(8),20-(R)-ginsenoside Rg2(9),20-(S)-ginsenoside Rh1(10),notoginsenoside B1(11),notoginsenoside ST13(12),3β,12β,25-trihydroxydammar-(E)-20(22)-ene-6-O-β-D-xylopyranosyl-(1→2)-β-D-glucopyranoside(13),notoginsenoside T5(14),20-(S)-ginsenoside Rg3(15)and 20-(R)-ginsenoside Rg3(16).Conclusion:Compound 1 was isolated from steamed Panax notoginseng for the first time,and its NMR data were supplemented.The inhibition rate of compound 7 on DHODH activity was 58.74%.

关 键 词：达玛烷型三萜皂苷 蒸制三七 分离鉴定 

分 类 号：R284.1[医药卫生—中药学]