γ射线诱变创制水稻突变体及其分子鉴定  

Creation of Rice Mutant by Gamma-ray and Its Molecular Identification

作　　者：李欣芃 张武汉[2] 张莉 舒服[2] 何强[2] 郭杨 邓华凤[2] 王悦[1] 孙平勇[2] LI Xin-peng;ZHANG Wu-han;ZHANG Li;SHU Fu;HE Qiang;GUO Yang;DENG Hua-feng;WANG Yue;SUN Ping-yong(College of Agronomy,Hunan Agricultural University,Changsha 410125;State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410125;Nuclear Agriculture and Space Breeding Research Institute,Changsha 410125)

机构地区：[1]湖南农业大学农学院,长沙410125 [2]湖南杂交水稻研究中心杂交水稻全国重点实验室,长沙410125 [3]湖南省核农学与航天育种研究所,长沙410125

出　　处：《生物技术通报》2025年第3期35-43,共9页Biotechnology Bulletin

基　　金：湖南省科技创新计划(2023NK2008);国家自然科学基金项目(32172000);湖南省农业科技创新资金(2023CX10);杂交水稻全国重点实验室基金(2023MS01)。

摘　　要：【目的】γ射线诱变具有效率高、方法简单等特点而广泛应用于水稻育种工作。OsGRF4是一个重要且稀有的水稻多效基因,能显著提高氮利用效率、产量和耐冷性,同时降低籽粒落粒性,旨在获得不同类型的突变体资源和挖掘OsGRF4的调控基因。【方法】利用γ射线对NIL-GRF4材料进行辐射诱变,通过功能标记和SSR标记对突变体的真实性和遗传背景进行鉴定。【结果】经过性状调查,获得15份粒型突变体、6份斑马叶突变体、4份分蘖突变体和3份不育突变体。其中一份重要的粒型突变体,其粒长达14.10 mm,粒长和千粒重比野生型分别增加1.50 mm和8.85 g,增幅分别为11.90%和23.95%。利用OsGRF4的功能标记和48对SSR标记鉴定了突变体的真实性和遗传背景,结果表明突变体材料均来源于野生型NIL-GRF4,并且突变体的遗传背景与野生型极为相似。【结论】已将粒型、斑马叶和不育突变体与野生型杂交构建了基因定位的F_(2)群体,为水稻育种提供重要的基因资源。【Objective】Gamma-ray mutagenesis has been widely used in rice breeding because of its high efficiency and simple method.OsGRF4 is an important and rare pleiotropic gene in rice,which can significantly improve nitrogen use efficiency,yield and cold tolerance,while reduce seed shattering,this work aims to obtain different types of mutant resources and mine the regulatory genes of OsGRF4.【Method】The rice material NIL-GRF4 was mutated byγrays in this study,the authenticity and genetic background of the mutants were identified by functional marker and SSR markers.【Result】By traits investigating,15 mutants of grain shape,6 mutants of zebra leaf,4 mutants of tiller and 3 mutants of sterile were obtained.Among them,an important grain type mutant was obtained.Its grain length reached 14.10 mm,and its grain length and 1000-grain weight increased by 1.50 mm and 8.85 g respectively compared with the wild type,with an increase of 11.90%and 23.95%respectively.The authenticity and genetic background of the mutants were identified by using the functional markers of OsGRF4 and 48 pairs of SSR markers.The results showed that the mutants were all derived from wild-type NIL-GRF4,and the genetic background of the mutants was very similar to that of wild-type NIL-GRF4.【Conclusion】The F2 populations have been constructed by crossbreeding the mutants of grain shape,zebra leaf and sterile with the wild type,providing important gene resources for rice breeding.

关 键 词：射线 OsGRF4 诱变育种 BSA-Seq 图位克隆 水稻 

分 类 号：S51[农业科学—作物学]