大豆GmPM31基因启动子响应高温高湿胁迫的功能分析  

Functional Analysis of Soybean GmPM31 Gene Promoter Involvement in Response to High Temperature and Humidity Stress

作　　者：林紫依 吴一舟 叶芳贤 朱淑颖 刘燕敏 刘骕骦 LIN Zi-yi;WU Yi-zhou;YE Fang-xian;ZHU Shu-ying;LIU Yan-min;LIU Su-shuang(Institute of Life and Health,Huzhou College,Huzhou 313000;Institute of Life and Environmental Sciences,Wenzhou University,Wenzhou 325000)

机构地区：[1]湖州学院生命健康学院,湖州313000 [2]温州大学生命与环境科学学院,温州325000

出　　处：《生物技术通报》2025年第3期90-97,共8页Biotechnology Bulletin

基　　金：国家级大学生创新创业训练计划资助项目(202313287004);湖州学院大学生创新创业训练科研项目(2023CXCY119,2023CXCY128)。

摘　　要：【目的】研究GmPM31启动子响应高温高湿胁迫过程中对种子活力形成的功能,为全面揭示大豆小热激蛋白参与高温高湿胁迫响应的功能奠定基础。【方法】对纯合T3代转GmPM31启动子拟南芥进行高温高湿胁迫(40℃/相对湿度100%)处理,以野生型拟南芥为对照,检测其耐受性以及气孔开度;通过GUS组织化学染色和实时荧光定量PCR检测分析大豆GmPM31启动子所驱动的GUS基因的表达情况;通过2,3,5-氯化三苯基四氮唑(TTC)染色法检测种子活力变化。【结果】与对照组相比,经高温高湿胁迫处理后的转大豆GmPM31启动子拟南芥对高温高湿的耐受性提高,GUS活性提高,在叶、根和花等中均有表达,发芽率以及种子活力增加。【结论】GmPM31启动子可提高种子对高温高湿胁迫的抗性和抗劣变的能力。【Objective】To study the function of GmPM31 promoter on seed vigor formation in response to high temperature and high humidity stress,and to lay a foundation for comprehensively unravelling the function of soybean small heat shock proteins involved in the response to high temperature and high humidity stress.【Method】Homozygous T3 transgenic Arabidopsis thaliana with GmPM31 promoter was treated with high temperature and high humidity stress(40℃/100%relative humidity),and wild-type A.thaliana was used as control to detect its tolerance and stomatal opening.Analysis of GUS gene expression driven by the soybean GmPM31 promoter by GUS histochemical staining and quantitative real-time PCR.The seed vigor was detected by 2,3,5-triphenyltetrazolium chloride(TTC)staining.【Result】Compared with the control group,the tolerance of GmPM31-transgenic promotor A.thaliana to high temperature and high humidity increased.The activity of GUS increased,and they were expressed in the leaves,roots and flowers,and germination rate and vigor increased.【Conclusion】GmPM31 promoter improve the resistance of seeds to high temperature and high humidity stress and the ability to resist deterioration.

关 键 词：大豆 启动子 高温高湿 种子活力 

分 类 号：Q94[生物学—植物学]