棉花SEC1复合体组分的鉴定与GhSCY1基因功能验证  

Identification of SEC1 Complex Components and Functional Validation of the GhSCY1 Gene in Cotton

作　　者：冯小康 梁倩 王学峰 孙杰[1] 薛飞[1] FENG Xiao-kang;LIANG Qian;WANG Xue-feng;SUN Jie;XUE Fei(College of Agriculture,Shihezi University,Key Laboratory of Oasis Eco-agriculture of Xinjiang Production and Construction Corps,Shihezi 832003)

机构地区：[1]石河子大学农学院新疆生产建设兵团绿洲生态农业重点实验室,石河子832003

出　　处：《生物技术通报》2025年第3期112-122,共11页Biotechnology Bulletin

基　　金：天山英才青年拔尖人才项目(2023TSYCJU0011);农业生物育种国家科技重大专项(2023ZD04038-02);石河子大学科技成果转化与技术推广专项(CGZH202312)。

摘　　要：【目的】SEC1复合体是位于类囊体上的蛋白质转运系统,由SCY1、SECE1和SECA1共同组成。SEC1系统参与类囊体蛋白的转运和整合,对维持叶绿体结构的稳定起着非常重要的作用。利用生物信息学从陆地棉全基因组中鉴定SEC1复合体基因,旨在为光合机制研究和棉花高产抗逆育种提供新的基因资源。【方法】基于拟南芥SEC1系统组分基因蛋白序列,从陆地棉基因组中鉴定棉花SEC1系统组分,分析该系统组分各基因的理化性质、跨膜结构、蛋白互作、启动子元件及表达模式。利用VIGS和透射电镜技术,研究叶绿体主要通道蛋白组分GhSCY1对叶绿素合成、叶绿体结构和形态的影响。【结果】在陆地棉中鉴定出了SEC1转运系统的组分,GhSCY1A/D、GhSECE1A/D和GhSECA1A/D。GhSCY1A/D含有1个SecY保守结构域,7个跨膜结构域,GhSECE1D只含有1个跨膜结构域。预测GhSCY1A/D与GhSECE1D在SEC1转运系统中共同形成叶绿体跨膜通道蛋白。GhSECA1A/D拥有1个SecA结构域,不含跨膜结构域,主要为蛋白转运过程提供能量。SEC1系统各基因在叶片中的表达量较高,且能够参与温度胁迫的响应。沉默GhSCY1基因(GhSCY1A和GhSCY1D)后,棉花叶片出现斑驳的淡黄色表型,叶绿素含量明显下降,叶片叶绿体结构和形态异常,没有淀粉粒累积。【结论】棉花SEC1系统分别由GhSCY1A/D、GhSECE1A/D和GhSECA1A/D构成,其中GhSCY1A/D基因在维持叶绿体结构和形态及叶绿素生物合成方面发挥重要作用。【Objective】The SEC1 complex is a protein transport system located on the thylakoid membrane,composed of SCY1,SECE1,and SECA1.The SEC1 system is involved in the transport and integration of thylakoid proteins,playing a very important role in maintaining the stability of chloroplast structure.Identifying SEC1 complex genes from the whole genome of upland cotton using bioinformatics aims to provide new gene resources for photosynthesis mechanism research and high-yield,stress-resistant cotton breeding.【Method】Based on the protein sequences of the Arabidopsis SEC1 system component genes,cotton SEC1 system components were identified from the upland cotton genome,and then the physicochemical properties,transmembrane structures,protein interactions,promoter elements,and expression patterns of these system components were analyzed.The effects of the main channel protein component GhSCY1 on chlorophyll synthesis,chloroplast structure,and morphology were studied using VIGS and transmission electron microscopy techniques.【Result】Components of the SEC1 transport system in upland cotton were identified,GhSCY1A/D,GhSECE1A/D,and GhSECA1A/D.GhSCY1A/D contains one SecY conserved domain and seven transmembrane domains,while GhSECE1D only contains one transmembrane domain.It is predicted that GhSCY1A/D and GhSECE1D together form the chloroplast transmembrane channel protein in the SEC1 transport system.GhSECA1A/D possesses a SecA domain and lacks transmembrane domains,mainly providing energy for the protein transport process.The expressions of the SEC1 system genes were high in the leaves and could participate in the response to temperature stress.After silencing the GhSCY1 genes(GhSCY1A and GhSCY1D),the cotton leaves demonstrated a mottled pale yellow phenotype,a significant decrease in chlorophyll content,abnormal chloroplast structure and morphology,and no starch granule accumulation.【Conclusion】The cotton SEC1 system is constituted by GhSCY1A/D,GhSECE1A/D,and GhSECA1A/D,among which the GhSCY1A/D genes play an im

关 键 词：棉花 SEC1系统 GhSCY1 叶色 叶绿体结构 温度胁迫 叶绿体蛋白转运 

分 类 号：Q94[生物学—植物学]