典型石化废水有机物组成特点及与生物毒性效应相关性分析  

Characteristics of organic compounds in typical petrochemical wastewater and the correlation with biotoxicity effects

作　　者：麻微微 康舒宇 黄虹溶 张晓琦 施雪卿 孔巧平 MA Weiwei;KANG Shuyu;HUANG Hongrong;ZHANG Xiaoqi;SHI Xueqing;KONG Qiaoping(School of Environmental and Municipal Engineering,Qingdao University of Technology,Qingdao 266520)

机构地区：[1]青岛理工大学,环境与市政工程学院,青岛266520

出　　处：《环境科学学报》2025年第2期110-120,共11页Acta Scientiae Circumstantiae

基　　金：山东省自然科学基金青年项目(No.ZR2021QE227);山东省高等学校青创科技支持计划(No.2022KJ164)。

摘　　要：石化废水毒性有机物组成复杂,其生物毒性效应及诱导生物毒性效应的关键有机物组分尚未明晰.本研究探究了石化废水中强极性、中极性和非极性有机物组成特点及与生物毒性效应之间的相关性.结果表明,石化进水和气浮工艺出水中强极性和非极性组分有机物含量均较高,经生化处理和深度处理后,最终出水各组分有机物均得到有效去除.由生物毒性分析可知,石化进水和气浮工艺出水中强极性组分具有较高的急性毒性,其EC50值分别可达5.48%和2.21%;而非极性组分具有较高的遗传毒性,其BEQ4-NQO值分别为132.64μg·L^(-1)和179.34μg·L^(-1).其中,氧化沟工艺出水各组分急性毒性降低50%以上,而深度处理工艺出水各组分遗传毒性降低60%以上,可见氧化沟工艺能够有效削减急性毒性,而深度处理工艺能够有效削减遗传毒性,但其最终出水仍对水环境具有潜在风险.石化废水强极性组分和非极性组分均显著导致嗜热四膜虫细胞产生过量活性氧自由基,导致其产生氧化应激反应,其中,急性毒性和遗传毒性均与氧化应激具有显著正相关性.Spearman相关性分析表明,石化废水中苯胺类、酚类、酮类和部分氮杂环类有机物与急性毒性和遗传毒性具有显著正相关性,可见这些物质是诱导生物毒性的主要有机物.以上研究阐明了石化废水中潜在毒性有机物组成及生物毒性特点,研究结果对于强化石化废水中关键毒性有机物的定向控制及保障水环境安全具有重要指导意义.The composition of toxic organics in petrochemical wastewater is complex,and the biotoxicity effects and key toxic organics have not been clarified.In this study,the characteristics of strong-polarity,mid-polarity and non-polarity organics in petrochemical wastewater and the correlation with biotoxicity were explored.The results showed that the concentrations of strong-polarity and non-polarity in influent and air flotation effluent were relatively high.After biochemical and advanced treatments,these organics were effectively removed in final effluent.Biotoxicity analysis showed that the strong-polarity organic in influent and air flotation effluent performed strong acute toxicity,with EC50 value of 5.48%and 2.21%,respectively.While the non-polarity organic performed strong genotoxicity,with BEQ4-NQO value of 132.64μg·L^(-1)and 179.34μg·L^(-1),respectively.Furthermore,acute toxicity of each component in oxidation ditch was decreased by more than 50%,and genotoxicity of each component in advanced treatment was decreased by more than 60%.It indicated that oxidation ditch could significantly reduce acute toxicity,and advanced treatment could effectively reduce genotoxicity.However,the final effluent still posed potential risks to the water environment.Moreover,both strong-polarity and non-polarity organics induced the production of excess reactive oxygen species in Tetrahymembrana thermophilic cells,causing oxidative stress responses.Both acute toxicity and genotoxicity were positively correlated with oxidative stress.Spearman correlation analysis showed that anilines,phenols,ketones and some nitrogen heterocyclic compounds were positively correlated with acute toxicity and genotoxicity,indicating that these organics played important role on inducing biotoxicity.The above results clarified the composition characteristic and biotoxicity effect of key toxic organics in petrochemical wastewater,which will provide important guidance for the targeted control of key toxic organics in petrochemical wastewater and for e

关 键 词：石化废水 急性毒性 遗传毒性 氧化应激 毒性有机物 

分 类 号：X703[环境科学与工程—环境工程] X171.5