激活线粒体自噬对草鱼脂肪细胞凋亡和生长发育的影响  

Effects of Activation of Mitophagy on Adipocyte Apoptosis and Growth and Development in Grass Carp Ctenopharyngodon idella

作　　者：曾彩虹 边晨晨 孙健[1] 吉红[1] 李汉东 胡泽超 ZENG Caihong;BIAN Chenchen;SUN Jian;JI Hong;LI Handong;HU Zechao(College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《水产科学》2025年第2期171-180,共10页Fisheries Science

基　　金：国家自然科学基金资助项目(32072989).

摘　　要：草鱼脂肪组织脂质过度沉积是水产养殖生产中常见的突出问题之一,而诱导脂肪细胞凋亡是降脂的有效手段。为探究草鱼脂肪细胞中线粒体自噬与细胞凋亡的关系,开展在体和离体两个试验。在水温24~30℃的在体试验中,将体质量约20 g的草鱼饲养在网箱中,投喂添加0(对照组)、10、20 mg/kg和40 mg/kg线粒体自噬激活剂羰基氰化物-3-氯苯腙56 d;离体试验中,分离原代草鱼脂肪细胞培养分化成熟后,用0(对照组)和20μmol/L羰基氰化物-3-氯苯腙处理细胞24 h。在体试验结果显示:与对照组相比,40 mg/kg线粒体自噬激活剂羰基氰化物-3-氯苯腙显著降低了草鱼腹腔脂肪指数(P<0.05)和甘油三酯含量(P<0.05),显著提高了自噬相关基因pink1、parkin、atg12、atg5和凋亡相关基因caspase3、caspase9的mRNA水平(P<0.05);与对照组相比,各个处理组的脂肪细胞面积大小无显著变化(P>0.05)。离体试验结果表明:羰基氰化物-3-氯苯腙处理极显著升高了细胞线粒体自噬相关基因pink1、parkin、atg4、atg7和atg12的mRNA水平(P<0.01)和细胞凋亡相关基因bax、caspase9的mRNA水平及bax/bcl-2比值(P<0.05或P<0.01),显著降低了抗凋亡基因bcl-2的mRNA水平(P<0.05)及细胞甘油三酯含量(P<0.05)。综合试验结果可知,激活草鱼脂肪细胞线粒体自噬可能会诱导脂肪细胞凋亡,降低草鱼腹腔脂质的蓄积。Excessive lipid deposition in grass carp Ctenopharyngodon idella adipose tissue as one of the common and prominent problems in aquaculture production can be dealt with induction of adipocyte apoptosis.To investigate the relationship between mitophagy and apoptosis in grass carp adipocytes,2 trails were designed and conducted in vitro and in vivo.In vitro,juvenile grass carp with body weight of some 20 g were reared in net cages and fed diets containing 0 mg/kg(control group),10 mg/kg,20 mg/kg,and 40 mg/kg mitophagy activatorcarbonyl cyanide-3-chlorophenylhydrazone(CCCP)at water temperature of 24—30℃for 8 weeks.In vitro trail,the isolated primary adipocytes of grass carpwere cultured,differentiated and matured,and then treated with CCCP at dose of 0(control group),and 20μmol/L for 24 h.The results in the vivo assay showed that compared with the control group,the treatment of 40 mg/kg of mitophagy activator led to significantly lower grass carp intraperitoneal fat index(P<0.05),and the content of triglycerides(P<0.05),and to significantly elevated the mRNA levels of autophagy-related genes pink1,parkin,atg12,atg5 and apoptosis-related genes caspase3 and caspase9(P<0.05),without significant change in the size of adipocyte area in each treatment group compared with the control group(P>0.05).In vivo results showed that there were significantly elevated cellular mitophagy-related genes pink1,parkin,atg4,atg7,and atg12(P<0.01),significant increase in the mRNA levels of the apoptosis-related genes bax,caspase9,and the bax/bcl-2 ratio(P<0.05 or P<0.01),and significant decrease in the mRNA of the anti-apoptosis gene bcl-2level(P<0.05),and cellular triglyceride content(P<0.05)in the fish fed the diet containing the activator.It was found that mitophagy activator treatment activated mitophagy and apoptosis.The findings indicate that reduction of intraperitoneal fat index in grass carp may be realized by inducing apoptosis of grass carp adipocytes,thus reducing lipid accumulation in grass carp.Combined in vivo and in vit

关 键 词：草鱼 脂质蓄积 脂肪细胞 线粒体自噬 细胞凋亡 PINK1/Parkin 

分 类 号：Q959.4[生物学—动物学]