乙酸钠添加对斜生栅藻处理紫菜加工废水效果的影响  

Effect of Sodium Acetate Supplementation on Nutrient Removal Efficiency of Laver Pyropia-Processing Wastewater by Microalga Scenedesmus obliquus

作　　者：王红岩 张怀 姜厚权 郭相旭 赵普 王欣淼 郑世燕 王强[4] WANG Hongyan;ZHANG Huai;JIANG Houquan;GUO Xiangxu;ZHAO Pu;WANG Xinmiao;ZHENG Shiyan;WANG Qiang(Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Key Laboratory of Marine Biotechnology,School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Co-Innovation Center of Jiangsu Marine Bio-Industry Technology,Jiangsu Ocean University,Lianyungang 222005,China;State Key Laboratory of Crop Stress Adaptation and Improvement,Henan University,Kaifeng 475004,China)

机构地区：[1]江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [2]江苏海洋大学海洋科学与水产学院,江苏省海洋生物技术重点实验室,江苏连云港222005 [3]江苏海洋大学,江苏省海洋生物产业技术协同创新中心,江苏连云港222005 [4]河南大学作物逆境适应与改良国家重点实验室,河南开封475004

出　　处：《水产科学》2025年第2期254-263,共10页Fisheries Science

基　　金：国家自然科学基金青年基金资助项目(32300321);江苏省自然科学基金青年项目(BK20191006);江苏省优势学科建设工程资助项目(PAPD);江苏省研究生科研与实践创新计划项目(SJCX23_1822).

摘　　要：为探究外加碳源对微藻处理紫菜加工废水效果的影响,选择斜生栅藻为试验对象,以乙酸钠为补充碳源,对比分析不同质量浓度(0、0.25、0.50、0.75、1.00、1.50g/L)乙酸钠作用下斜生栅藻在紫菜加工废水中的生长情况及其对废水中污染物的去除效果。试验结果显示,外源补充乙酸钠后废水的初始化学需氧量明显增加,但斜生栅藻对废水中的污染物仍表现出较好的去除效果(P<0.05)。培养5~7d,乙酸钠作用下斜生栅藻对废水中化学需氧量、总氮、总磷和藻胆蛋白的去除率最高,分别达81.97%、88.41%、94.10%和91.23%。培养结束后添加80mg/L壳聚糖对斜生栅藻进行絮凝采收,化学需氧量的最高去除率可提高至91.43%(P<0.05)。培养7d,斜生栅藻在1.50g/L乙酸钠质量浓度下的生物量和可溶性糖含量最高,分别为0.81g/L和168.61mg/g,与对照组相比分别提高了63.54%和1.06倍(P<0.05)。试验结果表明,在外源补充0.25~1.50g/L乙酸钠条件下,斜生栅藻可在紫菜加工废水中积累更高的生物量和可溶性糖含量,同时有效降低废水中的污染物含量,培养结束后添加适量壳聚糖进行絮凝可进一步提高化学需氧量去除率。To explore the impacts of carbon source supplementation on the purification efficiency of Pyropia-processing wastewater(PPW)by microalga,Scenedesmus obliquus was selected as the research object and sodium acetate as the supplementary carbon source.The growth performance of S.obliquus and the removal efficiency of pollutants in PPW were investigated under different sodium acetate concentrations(0,0.25,0.50,0.75,1.00,and 1.50 g/L).The results showed that the addition of exogenous sodium acetate led to an obvious increase in the initial concentration of chemical oxygen demand(COD)in PPW.However,S.obliquus was still effective in removing pollutants from PPW(P<0.05).After 5—7 d of culture,the maximal removal rate was shown to be 81.97%in COD,88.41%in total nitrogen(TN),94.10%in total phosphorus(TP),and 91.23%in phycobiliprotein(PP)from PPW by S.obliquus under different concentrations of sodium acetate.The addition of 80 mg/L chitosan to flocculate algal cells after culturing S.obliquus resulted in significant increase in the maximum COD elimination rate to 91.43%(P<0.05).The maximal biomass(0.81 g/L)and soluble sugar(168.61 mg/g)contents were observed in S.obliquus in the 1.50 g/L sodium acetate group for 7 days culture,63.54%and 1.06 times higher than those in the control group(P<0.05).The findings suggest that under exogenous supplementation of 0.25—1.50 g/L sodium acetate conditions,S.obliquus cultivated in PPW can accumulate higher biomass and soluble sugar content,with effectively reducing the levels of pollutants in PPW simultaneously.In addition,the COD removal rate is found to be further improved by adding moderate chitosan for flocculation at the end of microalgae culture.

关 键 词：斜生栅藻 紫菜加工废水 乙酸钠 污染物去除率 微藻生物量 

分 类 号：S963.21[农业科学—水产养殖]