大口黑鲈稚鱼赖氨酸需求量研究  

Requirement of Largemouth Bass Juveniles for Dietary Lysine

作　　者：朱喜锋 李诗洋 胡俊茹 梁浩辉 黄燕华 周萌 王国霞 ZHU Xifeng;LI Shiyang;HU Junru;LIANG Haohui;HUANG Yanhua;ZHOU Meng;WANG Guoxia(Collaborative Innovation Center of Aquatic Sciences,Key Laboratory of Animal Nutrition and Feed Science in South China Guangdong,Key Laboratory of Animal Breeding and Nutrition,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Innovative Institute of Animal Healthy Breeding,College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]广东省农业科学院动物科学研究所,农业农村部华南动物营养与饲料重点实验室,广东省畜禽育种与营养研究重点实验室,广东省农业科学院水产协同创新中心,广东广州510640 [2]仲恺农业工程学院动物科技学院,健康养殖创新研究院,广东广州510225

出　　处：《水产科学》2025年第2期295-302,共8页Fisheries Science

基　　金：广州市基础研究计划民生科技专题项目(202002020018);广东省重点领域研发计划项目(2021B0202050002).

摘　　要：为探究饲料赖氨酸水平对大口黑鲈稚鱼生长性能、消化和抗氧化能力的影响,在水温25~30℃下,将初始均质量约为0.07g的大口黑鲈放入75L的尼龙网箱中,饲养在室外养殖系统30d,每箱120尾,分别饲喂6种赖氨酸水平为1.31%、1.97%、2.64%、3.30%、3.97%和4.63%的等氮等能饲料,试验结束后测量各试验组大口黑鲈稚鱼的生长、消化和抗氧化指标。结果表明:赖氨酸水平1.31%和1.97%组大口黑鲈的存活率最低(P<0.05),随着饲料赖氨酸水平增加,试验鱼质量增加率和特定生长率先升后降,并在2.64%组达到最大值;赖氨酸水平2.64%组试验鱼胰蛋白酶活性、肠道绒毛高度、肌层厚度和杯状细胞数量达到最高值;各组鱼丙二醛含量和过氧化物酶活性呈先升后降的趋势,赖氨酸水平3.97%组达到最大值。以特定生长率为评价指标,通过折线模型分析得出,在此试验条件下大口黑鲈稚鱼赖氨酸需求量为2.65%,占饲料蛋白的5.19%。In order to probe the effects of diary lysine level on growth performance,digestion,and antioxidant capacity of juvenile largemouth bass Micropterus salmoides,juvenile largemouth bass with average body weight of approximately 0.07 g were reared in nylon cages with water volume of 75 L at stocking density of 150 individuals per cage disposed in an outdoor aquaculture system,and fed the isonitrogenous and isoenergetic diets containing lysine level of 1.31%,1.97%,2.64%,3.30%,3.97 and 4.63%for 30 days.The results showed that there was the minimal survival rates in the juvenile largemouth bass in 1.31%lysine groups and 1.97%lysine(P<0.05),with the first increase and then decrease in weight gain rate and specific growth rate in the juvenile largemouth bass fed the diets containing lysine,the maximal level in 2.64%lysine group,as the dietary lysine level increase.The maximal values for trypsin activity,intestinal villus height,muscular thickness,and the number of goblet cells were observed in the juvenile largemouth bass in 2.64%lysine group.The pattern of initial rise and followed by decline in content of malondialdehyde and the activity of peroxidase was found in the in the test fish,with the maximumt in the 3.97%lysine group.According to the specific growth rate as the evaluation index,largemouth bass juveniles had the lysine requirement of 2.65%,accounting for 5.19%of dietary protein,under this test condition based on the broken line model analysis.

关 键 词：赖氨酸 大口黑鲈 生长性能 抗氧化 肠道发育 

分 类 号：S965.199[农业科学—水产养殖]