不同生长速率单环刺螠幼体肠道菌群差异分析  

Difference Analysis in Intestinal Flora of Juvenile Echiura Worm Urechis unicinctus with Different Growth Rates

作　　者：董浩淼 王方轶 徐栋 焦绪栋[1,2] 王维忠 DONG Haomiao;WANG Fangyi;XU Dong;JIAO Xudong;WANG Weizhong(Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 100049,China;Weifang Fisheries Technology Extension Station,Weifang 261000,China;Shandong Blue Ocean Technology Co.,Ltd,Yantai 261400,China)

机构地区：[1]中国科学院烟台海岸带研究所,山东烟台264003 [2]中国科学院大学,北京100049 [3]潍坊市渔业技术推广站,山东潍坊261000 [4]山东蓝色海洋科技股份有限公司,山东烟台261400

出　　处：《水产科学》2025年第2期303-312,共10页Fisheries Science

基　　金：山东省水产种业领军企业项目(鲁农202351-21);烟台市双百计划产业领军人才项目(烟发改蓝色经济[2025]18号-1).

摘　　要：为探明单环刺螠生长速率差异与肠道菌群的关系,停食24h后在同一培育池内随机选择3块100cm2底质区域采集单环刺螠样本,体质量大于700mg的个体为快速生长组,体质量小于300mg的为慢速生长组,各组随机取9尾,经间氨基苯甲酸乙酯甲磺酸盐麻醉后无菌解剖取肠道,利用16S rRNA高通量测序分析肠道菌群的组成和变化。试验结果显示:慢速生长组肠道内菌群种类多于快速生长组;变形菌门和厚壁菌门是其肠道内优势菌门;快速生长组中罗尔斯通氏菌属相对丰度最高,显著高于慢速生长组,而慢速生长组中肝原体念珠菌属所占比例最大,显著高于快速生长组。LEfSe分析表明:快速生长组中含较多的假单胞菌属,推测有促进生长作用;慢速生长组中存在更多的肝原体念珠菌属,或是其生长速率过慢的标志属。功能预测显示,快速生长组中参与细胞过程和信号转导功能的菌群丰度显著高于慢速生长组,而在慢速生长组中参与细胞生长与死亡功能的群体占明显优势。试验结果表明,单环刺螠的生长速率与肠道菌群存在相关性,肠道菌群结构与功能的分析可为单环刺螠健康繁育和高效养成提供理论指导。In order to explore the relationship between growth difference of juvenile echiura worm Urechisunicinctus and its intestinal microbiota with different growth rates,the composition and changes in bacterial flora were analyzed in the intestines especially dissected of the juvenile echiura worm anesthesized by MS-222 with body weight of greater than 700 mg in fast-growth group(FG group)and with body weight of less than 300 mg in slow-growth group(SG group)sampled in three 100 cm2 substrate regions in the same culture pond by 16S rRNA high-throughput sequencing.The results showed that greater diversity of intestinal flora species was found in the juvenile echiura worm in FG group than that in FG group,with dominant bacteria in Proteobacteria and Firmicutes in the intestine.There was the maximal relative abundance of Ralstonia in FG group and significantly higher than that in SG group,while there was the maximal abundance of Candidatus_hepatoplasma in SG group and significantly higher than that in FG group.LEfSe analysis revealed that more Pseudomonas,which was speculated to promote individual growth,was observed in FG group,with more Candidatus_hepatoplasma in SG group,or the marker genus with too slow growth rate.Functional prediction showed that the relative abundance of bacteria involved in cellular processes and signaling was significantly higher in FG group than that in SG group,while the relative abundance of microorganisms involved in cell growth and death function groups accounted for a significant advantage in SG group,indicating that there was a correlation between the growth rate of echiura worm and the intestinal flora.The findings can provide theoretical guidance for the healthy breeding and efficient cultivation of echiura worm.

关 键 词：单环刺螠 生长速率 肠道菌群 16SrRNA 

分 类 号：S917.1[农业科学—水产科学]