柞蚕免疫蛹粉对中间球海胆生长和免疫的影响  

Effect of Tussah Immunoreactive Substances on Growth and Immunity of Sea Urchin Strongylocentrotus intermedius

作　　者：陈星合 贾心悦 温志新 李亚洁 王冬 都兴范 CHEN Xinghe;JIA Xinyue;WEN Zhixin;LI Yajie;WANG Dong;DU Xingfan(Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China;Liaoning Professional Technology Innovation Center of Further Processing and Utilization of Tussah Resources,Dalian 116024,China;Dalian Scitech Biotechnology Co.,Ltd,Dalian 116600,China)

机构地区：[1]辽宁省海洋水产科学研究院,辽宁大连116023 [2]辽宁省柞蚕资源深加工利用工程技术研究中心,辽宁大连116024 [3]大连升泰生物科技有限公司,辽宁大连116600

出　　处：《水产科学》2025年第2期313-319,共7页Fisheries Science

基　　金：财政部与农业农村部现代农业产业技术体系建设专项(CARS-18);辽宁省民生科技联合计划项目(2021JH2/10200039);辽宁省农业科学院重点学科资助项目(No.2022DD268646).

摘　　要：为探究柞蚕免疫蛹粉对中间球海胆生长和免疫的影响,在水温(19.0±0.1)℃下,将初始体质量(26.7±0.2)g/个的中间球海胆饲养于97L水槽中,每缸30个,投喂添加0%(对照组)、0.5%、1.0%和2.0%柞蚕免疫蛹粉的饲料,饲养60d,试验结束时测定生长性能和体腔液中免疫指标。试验结果显示:与对照组相比,饲喂0.5%和1.0%柞蚕免疫蛹粉组中间球海胆的特定生长率和壳径增长率均有所提高,但差异不显著,添加柞蚕免疫蛹粉各组中间球海胆的性腺指数均显著提高(P<0.05);1.0%柞蚕免疫蛹粉组中间球海胆性腺中鲜味氨基酸总量比对照组提高5.4%,不饱和脂肪酸中二十碳五烯酸和二十二碳六烯酸的含量提高10%以上;添加柞蚕免疫蛹粉各组体腔液中溶菌酶、超氧化物歧化酶及0.5%柞蚕免疫蛹粉组中间球海胆性腺中过氧化氢酶活性均显著提高(P<0.05)。综上,饲料中添加0.5%~1.0%的柞蚕免疫蛹粉可以促进中间球海胆的生长,同时提高其免疫力,为其在海胆配合饲料中的应用提供理论依据。To investigate the effect of tussah immunoreactive substances(TIS)on growth and immune function of sea urchin Strongylocentrotus intermedius,the sea urchin with initial body weight of(26.7±0.2)g were reared in a 97 L tank at stocking density of 30 individuals per tank,and fed the diets containing 0%(control group),0.5,1.0,and 2.0%of TIS for 60 days.At the end of the experiment,growth performance and immune indices were measured in body coelomic fluid.The results showed that the specific growth rate and shell diameter growth rate were found to be increased in the sea urchins in the 0.5%and 1.0%TIS-fed groups compared to the control group,without significant differences,and significant increase in the gonado-somatic index(P<0.05).The total amount of flavor amino acids was shown to be increased by 5.4%in the gonads of sea urchins in the 1.0%TIS group compared to the control group.Moreover,there was more than 10%increase in the content of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)in unsaturated fatty acids.The sea urchin had significant activities of lysozyme and superoxide dismutase in each TIS-supplemented group as well as the activity of catalase in the sea urchin gonads in the 0.5%dose group(P<0.05).In summary,diet supplemented with 0.5%—1.0%TIS can lead to enhance the growth and improve the immune response of the sea urchin.These findings offer a theoretical framework for integrating TIS into sea urchin formula feed.

关 键 词：中间球海胆 柞蚕免疫蛹粉 生长 免疫 

分 类 号：S968.9[农业科学—水产养殖]