基于一测多评法同时测定经典名方小陷胸汤中9种成分含量  

Simultaneous Determination of 9 Components in Xiaoxianxiong Decoction Based on QAMS Method

作　　者：刘园 宁致远 陆晨曦 陈志永 任慧[1] 任菲菲 杜霞[1] LIU Yuan;NING Zhiyuan;LU Chenxi;CHEN Zhiyong;REN Hui;REN Feifei;DU Xia(Institute of traditional Chinese Medicine,Shaanxi Academy of Traditional Chinese Medicine,Xi'an 710061,China;State Key Laboratory for Quality Research of Chinese Medicines,Macao University of Science and Technology,Macao 999078,China)

机构地区：[1]陕西省中医药研究院中药研究所,西安710061 [2]澳门科技大学中药质量研究国家重点实验室,中国澳门999078

出　　处：《中国药学杂志》2025年第2期172-179,共8页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金青年科学基金项目资助(82204711);陕西省中医药管理局“双链融合”中青年科研创新团队项目资助(2022-SLRH-YQ-003);陕西省中医药管理局“医研校企”中医药传承创新平台项目资助。

摘　　要：目的建立一测多评法(quantitative analysis of multi-components by single marker,QAMS)同时测定小陷胸汤中次黄嘌呤、香草酸、木兰花碱、去亚甲基小檗碱、非洲防己碱、药根碱、盐酸黄连碱、盐酸巴马汀、盐酸小檗碱的含量。方法本实验采用色谱柱Spursil C18(4.6 mm×250 mm,5μm),以乙腈-0.05 mol·L-1磷酸二氢钾溶液加磷酸调pH=4.0为流动相,检测波长:0~20 min:290 nm;20~45 min:300 nm,柱温30℃,进样量10μL。以盐酸小檗碱为内参物,分别计算其余8个成分的相对校正因子并计算各成分含量,比较外标法(external standard method,ESM)和QAMS法结果的差异。结果小陷胸汤9个成分在一定浓度范围与峰面积呈良好的线性关系(r>0.9980),平均加样回收率为94.36%~105.57%,相对标准偏差(relative standard deviation,RSD)为0.52%~3.48%,QAMS法与ESM法测定的结果无显著性差异。结论本研究建立的小陷胸汤QAMS法结果准确可靠,该方法简便易行,可用于小陷胸汤的质量控制。OBJECTIVE To establish a quantitative analysis of multi-components by single marker(QAMS)for the simultaneous determination of hypoxanthine,vanillic acid,magnolithine,demethyleneberberine,Columbamine,Jatrorrhizine hydrochloride,coptisine hydrochloride,palmatine hydrochloride and berberine hydrochloride in Xiaoxianxiong decoction.METHODS The separation was performed on a Spursil C18 column(4.6 mm×250 mm,5μm)with acetonitrile and 0.05 mol·L-1 potassium dihydrogen phosphate solution plus phosphoric acid adjusted to pH=4.0 as the mobile phases.Detection wavelength:0-20 min:290 nm;20-45 min:300 nm,column temperature 30℃,injection volume 10μL.Using berberine hydrochloride as the internal reference material,the relative correction factors of the remaining eight components were calculated and the content of each component was calculated,and the results were compared with those of the external standard method(ESM)and the QAMS method.The differences between the results of the external standard method(ESM)and QAMS method were compared.RESULTS The nine components of Xiaoxianxiong decoction showed a good linear relationship with peak area in a certain concentration range(r>0.9980),with the average spiked recoveries of 94.36%-105.57%and the RSD was 0.52%-3.48%,and the results of component contents determined by QAMS and ESM were not statistically significant.CONCLUSION The results of the established QAMS for Xiaoxianxiong decoction are accurate and reliable,and the method is simple and easy to be applied to the quality control of Xiaoxianxiong Decoction.

关 键 词：小陷胸汤 质量分析 一测多评法 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]