DNA-free base editing in lettuce via in vitro transcribed base editors  

作　　者：Eunbin Lee Yunsun Kim Minju Kim Donghui Lee Beum-Chang Kang 

机构地区：[1]Department of Horticulture,College of Agricultural Life Science,Jeonbuk National University,Jeonju 54896,Korea

出　　处：《Journal of Integrative Plant Biology》2025年第2期199-201,共3页植物学报(英文版)

基　　金：supported by the National Research Foundation of Korea(NRF)grant funded by the Korea Government(MSIT)(No.RS-2022NR072144);the Rural Development Administration under the New Breeding Technologies Development Program(No.RS-2024-00322224)。

摘　　要：Recent advances with DNA-free clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated nuclease 9(Cas9)methods using ribonucleoproteins(RNPs)in protoplasts(Woo et al.,2015)or RNAs in callus(Zhang et al.,2016)can effectively modify the plant genome with minimal off-target effects and non-integrated DNA fragments,making them attractive for crop development(Chen et al.,2019).Base editors are composed of Cas9 nickase and adenine or cytosine deaminases to generate precise base conversions(A-to-G or C-to-T)at the desired target site(Anzalone et al.,2020).

关 键 词：DNA BASE free 

分 类 号：Q78[生物学—分子生物学]