RBB1 negatively regulates rice disease resistance by modulating protein glycosylation  

作　　者：Bin Zhang Mingliang Guo Xiangpei Liu Bintao Zhang Yan Cui Xinglan Cao Zhipeng Zhang Chuanlin Shi Hua Wei Huiying He Hong Zhang Yiwang Zhu Xianmeng Wang Yang Lv Xiaoman Yu Dandan Chen Qiaoling Yuan Sheng Teng Tongjun Sun Qian Qian Lianguang Shang 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518124,China [2]Yazhouwan National Laboratory,No.8 Huanjin Road,Yazhou DistrictSanya City,Hainan Province 572024,China [3]Key Laboratory of Microbiological Metrology,Measurement&Bio-product Quality Security,State Administration for Market Regulation,College of Life Sciences,China Jiliang University,Hangzhou 310018,China [4]State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou,Zhejiang 310000,China [5]Nanfan Research Institute,Chinese Academy of Agriculture Science,Sanya,Hainan 572024,China

出　　处：《Journal of Integrative Plant Biology》2025年第2期391-407,共17页植物学报(英文版)

基　　金：supported by the Biological Breeding-National Science and Technology Major Project(2023ZD04073);the Guangdong Basic and Applied Basic Research Foundation(2021A1515111123,2023A1515110409);the China Postdoctoral Science Foundation(2020M683000);the National Natural Science Foundation of China(32201788)。

摘　　要：Glycosylation,a prevalent post-translational modification in eukaryotic secreted and membraneassociated proteins,plays a pivotal role in diverse physiological and pathological processes.Although UDP-N-acetylglucosamine(UDP-Glc NAc)is essential for this modification,the specific glycosylation mechanisms during plant leaf senescence and defense responses remain poorly understood.In our research,we identified a novel rice mutant named rbb1(resistance to blast and bacterial blight1),exhibiting broad-spectrum disease resistance.This mutant phenotype results from a loss-of-function mutation in the gene encoding glucosamine-6-phosphate acetyl-transferase,an important enzyme in D-glucosamine6-phosphate acetylation.The rbb1 mutant demonstrates enhanced defense responses,evident in increased resistance to rice blast and bacterial blight,along with the upregulation of defense-response genes.Various biochemical markers indicate an activated defense mechanism in the rbb1 mutant,such as elevated levels of reactive oxygen species and malondialdehyde,reduced enzyme activity and UDPGlc NAc content,and decreased expression of Nglycan and O-glycan modifying proteins.Moreover,proteome analysis of N-glycosylation modifications reveals alterations in the N-glycosylation of several disease-resistance-related proteins,with a significant reduction in Prx4 and Prx13 in rbb1-1.Additionally,the knockout of Prx4 or Prx13 also enhances resistance to Xanthomonas oryzae pv.oryzae(Xoo)and Magnaporthe oryzae(M.oryzae).This study uncovers a novel mechanism of defense response in rice,suggesting potential targets for the development of disease-resistant varieties.

关 键 词：crop genetics molecular biology disease resistance glycosylation modification 

分 类 号：S51[农业科学—作物学]