ε-聚赖氨酸盐酸盐对大黄鱼优势腐败菌的抑制作用  

The antibacterial effects ofε-polylysine hydrochloride against predominant spoilage bacteria from large yellow croaker

作　　者：张卫斌[1,2] 陈帅[1,2] 石双妮[1,2] 叶知秋 ZHANG Weibin;CHEN Shuai;SHI Shuangni;YE Zhiqiu(School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou,Zhejiang 310018,China;Zhejiang Province Key Laboratory of Food Safety,Zhejiang Gongshang University,Hangzhou,Zhejiang 310018,China)

机构地区：[1]浙江工商大学食品与生物工程学院,浙江杭州310018 [2]浙江工商大学浙江省食品安全重点实验室,浙江杭州310018

出　　处：《食品与机械》2025年第1期133-139,共7页Food and Machinery

基　　金：浙江省自然科学基金重点项目(编号:LZ23C200002)。

摘　　要：[目的]探究ε-聚赖氨酸盐酸盐对大黄鱼源波罗的海希瓦氏菌和荧光假单胞菌的抑制作用。[方法]通过测定最小抑菌浓度、生长曲线、菌液电导率、碱性磷酸酶活性、紫外吸收物质、生物膜生成抑制率、菌落总数以及挥发性盐基氮等指标分析ε-聚赖氨酸盐酸盐对波罗的海希瓦氏菌和荧光假单胞菌生长情况、生物膜破坏情况及致腐能力的影响。[结果]ε-聚赖氨酸盐酸盐对波罗的海希瓦氏菌和荧光假单胞菌的最小抑菌浓度分别为0.250,0.125 mg/mL;经ε-聚赖氨酸盐酸盐处理后,波罗的海希瓦氏菌和荧光假单胞菌的生长被明显抑制,电导率、AKP酶活性以及紫外吸收物质泄漏明显增加,并呈质量浓度依赖性;接种了波罗的海希瓦氏菌和荧光假单胞菌的鱼块其细菌总数和TVB-N值明显下降。[结论]ε-聚赖氨酸盐酸盐能破坏波罗的海希瓦氏菌和荧光假单胞菌细胞壁,增强细胞膜的通透性,抑制生长和影响致腐能力。[Objective]To investigate the antibacterial effect ofε-polysine hydrochloride on Shewanella baltica and Pseudomonas fluorescens derived from large yellow croaker.[Methods]The antibacterial effects ofε-polylysine hydrochloride on the growth of Shewanella baltica and Pseudomonas fluorescens was analyzed by the minimum inhibitory concentration and the growth curve.By measuring the bacterial fluid conductivity,alkaline phosphatase activity,ultraviolet absorbent substances and biofilm formation inhibition rate,the cell integrity and permeability were evaluated.The aerobic plate count and total volatile basic nitrogen of the flesh of large yellow croaker inoculated with spoilage bacteria were determined the effect ofε-polylysine hydrochloride on the spoilage ability of Shewanella baltica and Pseudomonas fluorescens.[Results]The minimum inhibitory concentrations ofε-polylysine hydrochloride against Shewanella baltica was 0.250 mg/mL,while Pseudomonas fluorescein was 0.125 mg/mL.With the treatment ofε-polylysine hydrochloride,the growth of Shewanella baltica and Pseudomonas fluorescein was significantly inhibited,and the conductivity,AKP enzyme activity and UV absorbent leakage were significantly increased in a mass concentration dependent manner.Fish inoculated with Shewanella baltica and Pseudomonas fluorescein showed a significant decrease in total bacterial count and TVB-N values.[Conclusion]ε-polylysine hydrochloride can destroy the cell integrity of Shewanella baltica and Pseudomonas fluorescein,inhibit the growth and affect the spoilage ability.

关 键 词：ε-聚赖氨酸盐酸盐 波罗的海希瓦氏菌 荧光假单胞菌 抑菌机制 

分 类 号：TS2[轻工技术与工程—食品科学与工程]