武汉地区单试剂反应性献血者归队策略及结果研究  

Study on Reentry Strategy and Results of Blood Donors with Single Reagent Reactivity in Wuhan Area

作　　者：许婷婷[1] 余琴[1] 柯颂庆 蔡雁[1] 谢松丽[1] 熊晶 赵磊[1] XU Ting-Ting;YU Qin;KE Song-Qing;CAI Yan;XIE Song-Li;XIONG Jing;ZHAO Lei(Wuhan Blood Center,Wuhan 430030,Hubei Province,China)

机构地区：[1]武汉血液中心,湖北武汉430030

出　　处：《中国实验血液学杂志》2025年第2期530-537,共8页Journal of Experimental Hematology

基　　金：中国输血协会威高科研基金资助项目(CSBT-WG-2022-03)。

摘　　要：目的:对武汉地区执行归队策略的献血者的检测结果、再次献血情况、特征进行研究,探讨献血者归队策略的合理性、有效性,为归队流程的改进提供数据支撑。方法:对2020年1月到2023年12月符合武汉血液中心归队条件并主动申请归队的献血者实施归队检测并对结果进行分析。按照归队策略,血清学检测和核酸检测平行进行,血清学检测使用两个不同厂家试剂进行ELISA初筛,初筛反应性样本进行双孔复试,HBV/HCV/HIV核酸检测采用RT-PCR单检模式。对既往HBV单试剂反应性归队献血者补充HBcAb化学发光检测,TP单试剂反应性归队献血者若归队检测呈单试剂反应性则补充TP抗体确证试验,既往HIV单试剂反应性归队献血者若归队检测呈单试剂反应性则送疾控中心进行确证实验。结果:符合归队条件献血者7098名,其中716名主动申请归队,436名成功归队,251名进入下一轮归队流程,29名不能归队。各年度归队者符合解除献血屏蔽要求的比率分别为66.67%(42/63)、54.73%(81/148)、60.71%(136/224)、62.99%(177/281),截止到2023年12月31日,归队成功的献血者中有275名再次参加献血,各年度再次献血比率分别为76.19%(32/42)、72.84%(59/81)、61.76%(84/136)、56.50%(100/177),献血后有31名献血者再次不合格永久屏蔽。归队结果在屏蔽项目、文化程度、年龄、是否结婚上的差异有统计学意义(P<0.05)。结论:目前采供血机构实施的归队策略能够有效召回部分献血者,减少献血者负面情绪,增加血液资源。Objective:To study the results,re-donation situation and characteristics of single-reagent reactive blood donors who were put into the reentry strategy in Wuhan area,explore the rationality and effectiveness of the current reentry strategy,and provide data support for the improvement of the reentry process of blood donors.Methods:From January 2020 to December 2023,blood donors who conform the reentry criteria and voluntarily applied for returning to Wuhan Blood Center were tested and the results were analyzed.According to the reentry strategy,serological testing and nucleic acid testing were carried out in parallel,serological testing was performed by ELISA with reagents from two different manufacturers,and the primary reactive samples were tested by double-well retest,and HBV/HCV/HIV nucleic acid detection was performed by RT-PCR with an individual donor test mode.Supplementary HBcAb testing was applied for HBV single reagent reactivity by chemiluminescence method.Supplementary TP-WB testing was applied for returning blood donors with repeated TP single reagent reactivity.If returning blood donors with HIV single reagent reactivity were repeated single reagent reactivity,the samples were sent to local CDC for confirmatory test.Results:7098 blood donors were qualified for reentry,716 donors voluntarily applied for reentry,436 donors successfully reentry,251 donors entered the next round,29 donors could not reentry.The reentry rates for the past four years were 66.67%(42/63),54.73%(81/148),60.71%(136/224)and 62.99%(177/281),respectively.Up to December 31,2023,275 donors donated blood again,and the donation rates for past four years were 76.19%(32/42),72.84%(59/81),61.76%(84/136)and 56.50%(100/177),respectively.After donating blood,31 donors were disqualified again by blood screening and subjected to permanent deferral.The results of returning to the team had statistical differences in reentry items,educational level,age,and marriage(P<0.05).Conclusion:The current reentry strategy adopted by the blood donation and

关 键 词：献血 献血者归队 归队策略 补充实验 确证实验 

分 类 号：R446.6[医药卫生—诊断学] R457.1[医药卫生—临床医学]