甘肃省东乡族12个稀有血型系统基因多态性初探  

A Preliminary Study on Genetic Polymorphism of 12 Rare Blood Group of Dongxiang Nationality in Gansu Province

作　　者：丁佳栋 王艺媛 张晓萍[1] DING Jia-Dong;WANG Yi-Yuan;ZHANG Xiao-Ping(Department of Blood Transfusion,Gansu Provincial Hospital,Lanzhou 730000,Gansu Province,China)

机构地区：[1]甘肃省人民医院输血科,甘肃兰州730000

出　　处：《中国实验血液学杂志》2025年第2期552-556,共5页Journal of Experimental Hematology

基　　金：甘肃省科技计划项目(23JRRA1781)。

摘　　要：目的:对甘肃东乡族人群Rh、MNS、Duffy、Kidd、Kell、Diego、Dombrock、Yt、Colton、Scianna、Lutheran、Lw12个红细胞血型系统的等位基因进行检测,了解东乡族人群常见的稀有血型等位基因的特点,为安全输血和血型基因库的建立提供依据。方法:采用荧光PCR法,对100位甘肃东乡族人群12个红细胞血型系统的等位基因进行分型,并分析基因频率与中国其他地区的差异。结果:甘肃东乡族人群的Rh、MNS、Dombrock血型系统的基因频率类似北方地区;Duffy血型系统存在特殊性,低于南方大部分地区而小于北方地区;Kidd、Kell、Diego血型系统与中国其他地区无异;Lutheran血型系统的Lu^(a)基因频率高于中国所有地区,可能存在遗传变异或与样本的选择与数量有关。Yt、Colton、Scianna、Lw血型基因呈现单态性分布,基因型分别为Yt^(a)Yt^(a)、Co^(a)Co^(a)、Sc1Sc1、Lw^(a)Lw^(a)。结论:Rh、MNS、Duffy、Kidd、Kell、Diego、Dombrock、Lutheran血型系统呈现多态性分布,Yt、Colton、Scianna、Lw血型基因呈现单态性分布,甘肃东乡族血型基因分布存在其特殊性。Objective:To detect the alleles of 12 blood group systems(Rh,MNS,Duffy,Kidd,Kell,Diego,Dombrock,Yt,Colton,Scianna,Lutheran and Lw)of Dongxiang ethnic group in Gansu province,and understand the characteristics of rare blood group alleles common in Dongxiang ethnic group,in order to provide a basis for safe blood transfusion and the establishment of blood group gene bank.Methods:The alleles of 12 blood group systems were classified by polymerase chain reaction(PCR)in 100 people from Dongxiang ethnic group in Gansu province,and the differences of gene frequency compared to other areas in China were analyzed.Results:The allele frequencies of Rh,MNS,and Dombrock blood group systems of Dongxiang ethnic group in Gansu province were similar to northern regions.The Duffy blood group system exhibited specificity,with frequencies lower than most southern regions as well as northern regions.There were no significant differences in Kidd,Kell and Diego blood group systems compared to other regions in China.The Lu^(a)gene frequency of Lutheran blood group system was higher than all regions in China,which might be associated with genetic variation or sample selection and size.Yt,Colton,Scianna and Lw blood group genes showed monomorphic distribution,and the genotypes were Yt^(a)Yt^(a),Co^(a)Co^(a),Sc1Sc1 and Lw^(a)Lw^(a),respectively.Conclusion:Rh,MNS,Duffy,Kidd,Kell,Diego,Dombrock and Lutheran blood group systems show polymorphic distribution,while Yt,Colton,Scianna and Lw blood group systems show monomorphic distribution.The distribution of blood group genes among Dongxiang ethnic group in Gansu province has its own specificity.

关 键 词：东乡族 群体遗传学 基因库 稀有血型 

分 类 号：R457.11[医药卫生—治疗学]