基于实验验证和网络药理学探讨辛夷治疗变应性鼻炎的作用机制  

The Mechanism of Xinyi in the Treatment of Allergic Rhinitis Based on Experimental Verification and Network Pharmacology

作　　者：陈屿 武新亮 赵振宇[4] 肖斌 张娜[1] CHEN Yu;WU Xinliang;ZHAO Zhenyu;XIAO Bin;ZHANG Na(Department of Pharmacy,Baotou Medical College,Baotou 014040,Inner Mongolia,China;General Clinical Research Center,Ordos Central Hospital,Ordos 017000,Inner Mongolia,China;Department of Pharmacy,Tianjin Medical University Baodi Hospital,Tianjin 301800,China;Tianjin Medical University Chu Hsien-I Memorial Hospital/Tianjin endocrinology institute/National Health Commission Key Laboratory of Hormone and Development/Tianjin Key Laboratory of Metabolic Diseases,Tianjin 300134,China)

机构地区：[1]内蒙古科技大学包头医学院药学院,内蒙古包头014040 [2]鄂尔多斯市中心医院中心实验室,内蒙古鄂尔多斯017000 [3]天津医科大学附属宝坻医院药剂科,天津301800 [4]天津医科大学朱宪彝纪念医院/天津市内分泌研究所/国家卫健委激素与发育重点实验室/天津市代谢性疾病重点实验室,天津300134

出　　处：《中国药物滥用防治杂志》2025年第1期41-45,共5页Chinese Journal of Drug Abuse Prevention and Treatment

基　　金：天津市卫生健康委员会、天津市中医药管理局中医中西医结合科研课题(编号:2021021);国家自然科学基金地区基金项目(编号:82260822)。

摘　　要：目的:探讨辛夷通过抑制肥大细胞脱颗粒治疗变应性鼻炎(AR)的作用机制。方法:将P815细胞分为对照组(nc)、模型组(mc)、阳性药组(pc)及辛夷水提物低(0.01 mg/ml)、中(0.1 mg/ml)、高(1 mg/ml)剂量组,检测其对P815肥大细胞脱颗粒的抑制作用。利用TCMSP、UniProt、SwissTargetPrediction数据库及文献查阅等检索辛夷的活性成分和相关靶点;利用GeneCards、OMIM、MelaCards、TTD数据库获取AR的疾病靶点。构建共同靶点蛋白相互作用网络(PPI),对关键作用靶点进行GO功能和KEGG通路富集分析。结果:辛夷对P815细胞脱颗粒具有明显的抑制作用(P<0.05)。成分靶点370个,肥大细胞脱颗粒靶点1940个,AR靶点2740个,共同靶点90个,其中TNF、SRC、AKT、STAT3、EGFR为关键靶基因。GO富集分析发现炎症应答、磷酸化、蛋白质磷酸化、质膜等参与AR的发生与治疗。KEGG通路富集分析显示,与EGFR酪氨酸激酶抑制剂耐药性、胰腺癌、非小细胞肺癌、fcεRI信号通路密切相关。结论:辛夷能抑制肥大细胞脱颗粒发并通过多靶点途径发挥治疗AR的作用。Objective:To explore the mechanism of Xinyi in the treatment of allergic rhinitis(AR)by inhibiting mast cell degranulation.Methods:P815 cells were divided into control group(nc),model group(mc),positive drug group(pc)and low(0.01 mg/mL),medium(0.1 mg/mL)and high(1 mg/mL)dose groups of Xinyi water extract to detect its inhibitory effect on P815 mast cell degranulation.The active components and related targets of Xinyi were searched by TCMSP,UniProt,SwissTargetPrediction database and literature review.The disease targets of AR were obtained by using GeneCards,OMIM,MelaCards and TTD databases.A common target protein interaction network(PPI)was constructed,and GO function and KEGG pathway enrichment analysis were performed on key targets.Results:Xinyi had a significant inhibitory effect on the degranulation of P815 cells(P<0.05).There were 370 component targets,1940 mast cell degranulation targets,2740 AR targets,and 90 common targets,of which TNF,SRC,AKT,STAT3,and EGFR were key target genes.GO enrichment analysis found that inflammatory response,phosphorylation,protein phosphorylation,and plasma membrane were involved in the occurrence and treatment of AR.KEGG pathway enrichment analysis showed that it was closely related to EGFR tyrosine kinase inhibitor resistance,pancreatic cancer,non-small cell lung cancer,and fcεRI signaling pathway.Conclusion:Xinyi can inhibit mast cell degranulation and exert a therapeutic effect on AR through multiple target pathways.

关 键 词：辛夷 网络药理学 变应性鼻炎 肥大细胞 作用机制 

分 类 号：R285[医药卫生—中药学]