基于环境病毒检测仪的量子点免疫层析试纸条的研究  

Research on quantum-dot immunochromatographic test strip based on environmental virus detector

作　　者：吴向阳 郑璐璐 WU Xiangyang;ZHENG Lulu(School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学光电信息与计算机工程学院,上海200093

出　　处：《光学仪器》2025年第1期58-64,共7页Optical Instruments

摘　　要：近年来甲型H1N1流感、埃博拉、乙型流感、新冠病毒等突发的具有传染性的重大疫情,给人类的健康带来了很大影响,因此针对环境病毒的检测和预防变得至关重要。而免疫层析试纸条技术作为一种能够实现病毒快速检测的新兴技术,相较于酶联免疫吸附实验具有普适性和特异性强等优点,解决了传统的病毒抗原检测流程复杂、时间长等问题,同时也为环境监测提供了一种新的发展方向。然而,传统的免疫层析试纸条存在检测指标单一、检测限高等缺点。基于免疫层析试纸条和量子点荧光纳米微球,设计了一种量子点荧光免疫层析试纸条,它具有光稳定性好、亮度高和检测限低的特点。通过仪器配套的荧光检测模块进行分析和检测时,量子点荧光纳米微球的激发光可快速检测出环境中的病毒。该方法操作简单、数据稳定,为环境病毒检测提供了一种新思路。In recent years,major infectious outbreaks such as influenza A(H1N1),Ebola,influenza B,and novel coronavirus have had a huge impact on human health and safety,so the detection and prevention of environmental viruses is crucial.As an emerging technology that can rapidly detect viruses,immunochromatographic strip technology has the advantages of universality and strong specificity compared with ELISA,which solves the problems of complicated and long time of traditional viral antigen detection process,and also provides a new development direction for environmental monitoring.However,the traditional immunochromatographic test strips have the disadvantages of single detection index and high detection limit.Based on immunochromatographic strips and quantum dot fluorescence nanospheres,a quantum dot fluorescence immunochromatographic strip was designed in this study,which has the characteristics of good photostability,high brightness,and low detection limit.By using the fluorescence detection module of the supporting instrument for analysis and detection,viruses in the environment can be quickly detected based on the excitation light of quantum dot fluorescent nanospheres.The operation is simple and the data is stable,providing a new approach for environmental virus detection.

关 键 词：环境病毒检测 免疫层析技术 量子点荧光纳米微球 试纸条 

分 类 号：TB39[一般工业技术—材料科学与工程]