The impacts of PVC microplastics on physiology and transcriptomic responses of pearl oyster Pinctada fucata martensii  

作　　者：Robert MKUYE Luomin HUANG Chuangye YANG Xiuyan YANG Fortunatus MASANJA Salifu IBRAHIM Fenglan LU Shaojie ZHU Hailing WU Yuewen DENG 

机构地区：[1]Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China [2]Guangdong Science and Innovation Center for Pearl Culture,Zhanjiang 524088,China [3]Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province,Zhanjiang 524088,China [4]Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture,Zhanjiang 524088,China [5]Guangdong Marine Ecology Early Warning and Monitoring Laboratory,Zhanjiang 524088,China

出　　处：《Journal of Oceanology and Limnology》2025年第2期589-613,共25页海洋湖沼学报(英文)

基　　金：Supported by the Science and Technology Program of Guangdong Province(No.2022A1515010030);the National Natural Science Foundation of China(No.32102817);the Department of Education of Guangdong Province(Nos.2020ZDZX1045,2021KCXTD026);the earmarked fund for CARS-49,Students Innovation and Entrepreneurship Training Program(No.CXXL2022015);the Technology Program of Guangdong Zhanjiang(No.2022A01010);the Hengli Biosciences Excellence Project of Guangdong Ocean University(No.B23335-4)。

摘　　要：Microplastics(MPs),particularly polyvinyl chloride microplastics(PVC MPs)have become a notable environmental pollutant that affect various marine organisms such as Pinctada fucata martensii.As filter feeders,these bivalves consume significant volumes of water containing MPs,leading to contact with and ingestion of MPs.Moreover,given the ecological and economic importance of P.f.martensii in artificial pearl production,investigating the effects of PVC MPs exposure is crucial.This study aimed to investigate the effects of PVC MPs exposure on nucleus retention,pearl formation,oxidative stress by examining superoxide dismutase(SOD)activity,catalase(CAT)activity,lipid peroxidation(LPO),and total antioxidant capacity(TAOC)of P.f.martensii,while also exploring transcriptomic changes at different concentrations and exposure time points,including a recovery period.The pearl oysters were exposed to PVC MPs at concentrations of 1-,2.5-,and 5-mg PVC MPs/L for 10 d followed by 6 d of recovery.After 1-,4-,10-d of exposure,and additional 6 d of recovery,samples were taken and analyzed.Findings revealed that only prolonged exposure(10 d)to PVC MPs affected SOD activity,while CAT activity,LPO,and TAOC remained unaffected throughout the experiment.Notably,SOD activity was restored during the 6-d recovery phase.Transcriptome analysis highlighted significant gene alterations linked to various pathways,affecting cellular processes,environmental information processing,genetic information processing,metabolism,and organismal systems,with an increase in pathway-related genes during recovery,implying a potential role of PVC MPs as gene inhibitors.This study provided insights into the effects of PVC MPs on P.f.martensii,shedding light on pearl retention,oxidative systems,and molecular pathways influenced by PVC MPs.Additionally,it contributed novel information on potential MPs exposure biomarkers,particularly relevant to marine organisms like P.f.martensii.

关 键 词：microplastic(MP) Pinctada fucata martensii oxidative stress TRANSCRIPTOME 

分 类 号：X503.2[环境科学与工程—环境工程] X505