Advancing CRISPR base editing technology through innovative strategies and ideas  

作　　者：Xiongwei Fan Yang Lei Liren Wang Xiushan Wu Dali Li 

机构地区：[1]The Center for Heart Development,College of Life Science,Hunan Normal University,Changsha 410081,China [2]Shanghai Frontiers Science Research Baseof Genome Editing and Cell Therapy,Shanghai Key Laboratory of Regulatory Biology,Instituteof Biomedical Sciences and School of Life Sciences,East China Normal University,Shanghai 200241,China [3]Guangdong Provincial Key Laboratory of Pathogenesis,Targeted Prevention and Treatment of Heart Disease,Guangzhou 510100,China

出　　处：《Science China(Life Sciences)》2025年第3期610-627,共18页中国科学(生命科学英文版)

基　　金：supported by the National Natural Science Foundation of China(81970324);partially supported by grants from the National Key R&D Program of China 2023YFC3403400。

摘　　要：The innovation of CRISPR/Cas gene editing technology has developed rapidly in recent years.It is widely used in the fields of disease animal model construction,biological breeding,disease diagnosis and screening,gene therapy,cell localization,cell lineage tracking,synthetic biology,information storage,etc.However,developing idealized editors in various fields is still a goal for future development.This article focuses on the development and innovation of non-DSB editors BE and PE in the platform-based CRISPR system.It first explains the application of ideas for improvement such as“substitution”,“combination”,“adaptation”,and“adjustment”in BE and PE development and then catalogues the ingenious inversions and leaps of thought reflected in the innovations made to CRISPR technology.It will then elaborate on the efforts currently being made to develop small editors to solve the problem of AAV overload and summarize the current application status of editors for in vivo gene modification using AAV as a delivery system.Finally,it summarizes the inspiration brought by CRISPR/Cas innovation and assesses future prospects for development of an idealized editor.

关 键 词：CRISPR base editing prime editing INNOVATION 

分 类 号：Q78[生物学—分子生物学]