Comprehensive cultivation of the broiler gut microbiota guides bacterial isolation from chickens  

作　　者：Zhang-Chao Deng Ke-Xin Cao Yu-Xuan Huang Zhe Peng Ling Zhao Dan Yi Meng Liu Lv-Hui Sun 

机构地区：[1]State Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Frontiers Science Center for Animal Breeding and Sustainable Production,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan 430023,China

出　　处：《Science China(Life Sciences)》2025年第3期836-845,共10页中国科学(生命科学英文版)

基　　金：supported in part by the National Key Research and Development Program of China Projects(2022YFD1300402);fundamental research funds for the Central Universities(2662022DKPY002);the Top-Notch Young Talent Supporting Program(to LH Sun).

摘　　要：Chicken gut microbiota plays an important role in maintaining their physiological health.However,the cultivability of chicken gut microbiota is not well understood,limiting the exploration of certain key gut bacteria in regulating intestinal health and nutritional metabolism.This study aimed to examine the cultivability of chicken cecal microbiota and to provide guidance for future chicken gut microbiota cultivation.A total of 58 different culture conditions were applied to culture broiler cecal microbiota,and the culture-dependent(CD;pooled colonies form each plate)and culture-independent(CI;broiler cecal contents)samples were collected for 16S rRNA gene sequencing and microbial analysis.The CD methods detected higher microbial richness(3,636 vs 2,331 OTUs)than CI methods,and the recovery rates of bacterial OTUs and genera reached 43.6%and 68.9%,respectively.The genera of Bacteroides(19.9%),Alistipes(11.0%)and Barnesiella(10.7%)were highly abundant detected by CI methods,however,there occupied a small proportion(<1.0%)of total cultured microbiota in CD methods.We then developed reference figures and tables showing optimal cultivation conditions for different gut bacteria taxa.Moreover,81 different lactic acid bacteria strains covering 5 genera were isolated,and 15 strains had less than 97.0%similarity to known bacteria in the national center for biotechnology information(NCBI)online database.Overall,this study provides preliminary guidance in culturing specific gut microbiota from chickens,which will contribute to future studies to characterize the biological functions of key microbes in chicken nutritional metabolism and health.

关 键 词：CHICKEN gut microbes culturomics 16S rRNA sequencing lactic acid bacteria 

分 类 号：S831[农业科学—畜牧学]