Activatable red/near-infrared aqueous organic phosphorescence probes for improved time-resolved bioimaging  

作　　者：Yang Li Zhiqin Wu Zizhao Huang Chenjia Yin He Tian Xiang Ma 

机构地区：[1]Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Frontiers Science Center for Materiobiology and Dynamic Chemistry,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China

出　　处：《National Science Review》2025年第2期180-190,共11页国家科学评论(英文版)

基　　金：supported by the National Key Research and Development Program of China(2022YFB3203500);the National Natural Science Foundation of China(22125803,T2488302,22020102006 and 22307036);China Postdoctoral Science Foundation(2023M731079);the Fundamental Research Funds for the Central Universities.

摘　　要：Organic red/near-infrared(NIR)room-temperature phosphorescence(RTP)holds significant potential for autofluorescence-free bioimaging and biosensing due to its prolonged persistent luminescence and exceptional penetrability.However,achieving activatable red/NIR organic RTP probes with tunable emission in aqueous solution remains a formidable challenge.Here we report on aqueous organic RTP probes with red/NIR phosphorescence intensity and lifetime amplification.These probes consist of supramolecular assemblies comprising macrocyclic cucurbit[8]uril and amine-containing alkyl-bridged py ridiniums,ex hibiting viscosity-activatable phosphorescence with enhanced quantum yield(≤20%)and lifetime.Notably,by utilizing this activatable organic RTP probe,we successfully achieve two-photon imaging of lysosomal viscosity and mi l lisecond-scale time-resolved cell imaging.Moreover,intravital phosphorescence imaging by using an RTP probe enables the monitoring of viscosity variations in inflammatory mice,demonstrating a significantly improved signal-to-background ratio compared with fluorescence imaging.This activatable red/NIR supramolecular platform facilitates versatile high-resolution phosphorescence imaging for in vivo tracking of specific biomarkers and physiological events.

关 键 词：organic room-temperature phosphorescence activatable molecular probe HOST-GUEST time-resolved imaging in vivo biosensing 

分 类 号：O62[理学—有机化学]