硅缺乏调控小新月菱形藻中心碳代谢相关基因的表达  

Expressions of genes associated with sugar and carbon metabolism were regulated by silicon deficiency in Nitzschia closterium f.minutissima

作　　者：李亚军[1] 何金曼 张秀霞[1] 邓晓东[1] LI Yajun;HE Jinman;ZHANG Xiuxia;DENG Xiaodong(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bioresources,Haikou 571101,China)

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南省海洋生物资源功能性成分研究与利用重点实验室,海南海口571101

出　　处：《生物技术》2025年第1期25-29,94,共6页Biotechnology

基　　金：海南省自然科学基金高层次人才项目(322RC762,322RC766);农业农村部财政专项(NFZX2024)。

摘　　要：[目的]分析硅缺乏对小新月菱形藻基因在转录水平上的调控。[方法]收集缺硅24 h和正常培养条件下的藻细胞,利用illumina NovaSeq 6000平台对其RNA进行测序。以正常培养藻细胞为对照,鉴定硅缺乏条件下差异表达基因,并对其进行GO和KEGG显著性富集分析。[结果]以对照相比,共鉴定出668个基因和1306个基因在硅缺乏时分别上调和下调表达。GO显著性分析结果表明,差异表达基因显著性富集在38个GO条目中,主要与阴离子转运,膜组分及转录因子活性等相关。8个KEGG显著性富集通路主要与中心碳代谢等途径相关。进一步分析差异基因表达模式,发现与糖酵解相关基因均下调表达,碳代谢相关基因在硅缺乏时上调表达。[结论]硅缺乏诱导小新月菱形藻1974个基因在转录水平上差异表达,其中,糖代谢相关基因下调表达,而碳代谢相关基因上调表达。[Objective]To illustrate the transcriptome changes during silicon deficiency in Nitzschia closterium f.minutissima,The RNA-seq was performed to identify genes differentially expressed in silicon-deficient algal cells.[Method]The algal cells were collected after treatment with silicon deficiency for 24 hours and normal cultivation,and the RNA from the two samples were extracted and sequencing in illumina NovaSeq 6000 platform.The normal cells were used as control,the differential expressed genes in response to silicon deficiency were identified,and GO and KEGG enrichment analyses were performed.[Result]Compared with the control,668 genes were up-regulated and 1306 genes were down-regulated in silicon-deficient cells.The results of GO significant enrichment analysis showed that 38 GO terms such as anion transport,membrane component related and transcription factor activity were significantly enriched.In addition,eight KEGG pathways such as sugar and carbon metabolism were significantly changed after silicon deficiency.The further analysis of gene expression profiles showed that several genes involved in sugar metabolism were down-regulated,whereas genes related to carbon metabolism were up-regulated after silicon deficiency for 24 hours.[Conclusion]Silicon deficiency induced 1974 genes differentially expressed in N.closterium f.minutissima,among of which,genes related to sugar metabolism were down regulated and genes associated with carbon metabolism were up-regulated.

关 键 词：小新月菱形藻 硅缺乏 转录组测序 差异表达基因 GO功能分类 KEGG富集分析 中心碳代谢 基因调控 

分 类 号：Q175[生物学—水生生物学] Q945.1[生物学—普通生物学]