基于中红外光谱技术结合HPLC对不同产地地黄快速无损鉴别  

Rapid and Nondestructive Identification of Dihuang(Rehmannia glutinosa)of Different Origins Based on Infrared Spectroscopy and High Performance Liquid Chromatography

作　　者：乔璐[1,2] 阴紫钰 段浩瀚 谢赢赢 徐可可 刘永红 李菊香 董诚明 王磊[1] QIAO Lu;YIN Ziyu;DUAN Haohan;XIE Yingying;XU Keke;LIU Yonghong;LI Juxiang;DONG Chengming;WANG Lei(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China;Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials,Zhengzhou 450046,Henan,China;Beijing University of Chinese Medicine,Beijing 100105,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省道地药材生态种植工程技术研究中心,河南郑州450046 [3]北京中医药大学中药学院,北京100105

出　　处：《中华中医药学刊》2025年第3期42-46,I0003-I0009,共12页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点研发计划项目(2017YFC1702800);河南省重大科技专项项目(171100310500);河南省科技攻关基金项目(222102310182);河南省大学生创新创业训练计划项目(202410471017);河南中医药大学博士科研基金项目(BSJJ2014-01)。

摘　　要：目的对不同产地地黄的快速无损鉴别。方法采集四个省16个县区地黄样品的红外吸收光谱进行差异性分析,找出代表差异性的特征波段,利用高效液相色谱法的结果验证红外差异性分析的结果。结果166批样品的红外光谱峰形基本相似,在2928、1643、1419、1353、1238、1145、1053、925、799、700^543 cm^(-1)波位处有相同的吸收峰。其中河南省内3个地区的样品差异主要出现在1519^1494 cm^(-1);河北省内不同产地样品差异主要在1571和1020 cm^(-1)处;山西省内不同产地样品差异主要在1790^1642、1440、1030 cm^(-1);各省之间差异性比较中,山东省与其他各省具有明显差异,主要出现在1331、1582 cm^(-1);各县区之间的差异主要出现在1796、1718、1640、1606^1567、1492、1453、1277、1024 cm^(-1)。结论红外光谱结合高效液相色谱法可实现对不同产地地黄的快速无损鉴别。Objective To carry out the rapid and nondestructive identification of Dihuang(Rehmannia glutinosa)of different origins.Methods The infrared absorption spectrum of a total of 166 batches of Dihuang(Rehmannia glutinosa)in 16 counties and districts in four provinces was collected for differential analysis,and the results of the analysis were verified by high performance liquid chromatography.Results The infrared spectra of the 166 samples were basically similar in peak shape,with the same ab-sorption at 2928,1643,1419,1353,1238,1145,1053,925,799,700~543 cm^(-1).The differences between samples from the three sites in Henan Province were mainly at 1519~1494 cm^(-1).The differences between samples from different sites in Hebei Prov-ince were mainly at 1571 and 1020 cm^(-1).The differences between samples from different sites in Shanxi Province were mainly at 1790~1642,1440,1030 cm^(-1).Then among the differences between provinces,Shandong Province was significantly different from other provinces mainly at 1796,1718,1640,1606~1567,1492,1453,1277,1024 cm^(-1).Among of them,the main charac-teristic component in the band of 1640~1439 cm^(-1) was catalpol,and the strong absorption peak at 1052 cm^(-1) was C-O bond stretching vibration absorption in the structure of pistil side.Conlusion Infrared spectroscopy combined with HPLC can provide i-deas for the rapid and non-destructive identification of different origins of Dihuang(Rehmannia glutinosa).

关 键 词：地黄 中红外光谱 高效液相色谱法 不同产地 聚类分析 

分 类 号：R282.71[医药卫生—中药学]