一效膏促进糖尿病性溃疡愈合的抗焦亡机制研究  

Study on Anti-Pyroptosis Mechanism of Yixiao Ointment(一效膏)in Promoting The Healing of Diabetic Ulcer

作　　者：陈蕾 李大勇[2] CHEN Lei;LI Dayong(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2025年第3期189-193,I0035,I0036,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重大科技专项(2019YFC1709303)。

摘　　要：目的探讨一效膏通过抑制细胞焦亡促进糖尿病大鼠皮肤溃疡愈合的作用机制。方法72只SPF级雄性SD大鼠随机分为空白组、模型组,模型组大鼠以高脂高糖饲料喂养联合小剂量STZ腹腔注射的方法诱导糖尿病模型,1个月后各组大鼠分别切开皮肤造成开放创面构建糖尿病大鼠溃疡模型。造模成功后,将模型组分为模型对照组、一效膏组、表皮生长因子组,每组18只大鼠,分别连续14 d给予生理氯化钠注射液、一效膏或人表皮生长因子凝胶等干预后,观察皮肤溃疡创面愈合情况,采用酶联免疫吸附测定(Enzyme-Linked Immunosorbent Assay,ELISA)检测血清中白介素-1(IL-1)、白介素-18(IL-18)、肿瘤坏死因子-α(TNF-α)表达水平,采用Western blot及逆转录-聚合酶链反应(RT-PCR)法检测NOD样受体热蛋白结构域相关蛋白3(NLRP3)、半胱氨酸蛋白酶-1(Caspase-1)、核因子κB(NF-κB)、凋亡相关斑点样蛋白(ASC)蛋白水平及mRNA的表达。结果创面肉眼观察可发现一效膏组大鼠溃疡皮肤愈合明显。与模型对照组相比,一效膏组IL-1、IL-18、TNF-α水平显著下降,NLRP3、Caspase-1、NF-κB、ASC蛋白表达降低。结论一效膏能够促进糖尿病溃疡性皮肤愈合,其作用机制可能与其降低炎性反应,抑制细胞焦亡有关。Objective To investigate the mechanism of Yixiao Ointment(一效膏)in promoting the healing of skin ulcers in di-abetic rats by inhibiting pyroptosis.Methods Seventy-two male SD rats of SPF grade were randomly divided into blank group and model group,and the diabetes model was induced by high-fat and high-sugar chow combined with small-dose streptozoto-cin(STZ)intraperitoneal injection in the model group.One month later,the rats in each group were cut into the skin to create open wounds to construct the diabetic rat ulcer model.After successful modeling,the model group was divided into a model con-trol group(physiological sodium chloride injection),a Yixiao Ointment group(Yixiao Ointment)and an epidermal growth factor group(human epidermal growth factor gel)with 18 rats in each group.After intervention for 14 consecutive days,the healing of skin ulcerations was observed.Enzyme-linked immunosorbent assay(ELISA)was used for detecting the expression levels of in-terleukin-1(IL-1),interleukin-18(IL-18)and tumor necrosis factor-α(TNF-α)in serum,and the protein levels and mRNA expression levels of NOD-like receptor thermoprotein structural domain-associated protein 3(NLRP3),cysteine prote-ase-1(Caspase-1),nuclear factorκB(NF-κB),apoptosis-associated speck-like protein(ASC)were detected by Western blot and reverse transcription-polymerase chain reaction(RT-PCR).Results Visual observation of the ulcer surface showed that the ulcerated skin of rats in the Yixiao Ointment group healed significantly.Compared with those of the model control group,the levels of IL-1,IL-18 and TNF-αwere significantly decreased in the Yixiao Ointment group,and the protein expressions of NLRP3,Caspase-1,NF-κB and ASC were decreased in the Yixiao Ointment group.Conclusion Yixiao Ointment can promote the healing of diabetic ulcerated skin,and its mechanism of action may be related to its ability to reduce the inflammatory re-sponse and inhibit cellular death.

关 键 词：糖尿病性溃疡 一效膏 炎性反应 细胞焦亡 

分 类 号：R255.4[医药卫生—中医内科学]