血清miR-217对动脉粥样硬化的辅助诊断价值评估  

Evaluation of the auxiliary diagnostic value of serum miR-217 for atherosclerosis

作　　者：池家欣 程雨林 孙静芳 李欣莹 罗晨 王婉 CHI Jiaxin;CHENG Yulin;SUN Jingfang;LI Xinying;LUO Chen;WANG Wan(School of Medical Technology,Xuzhou Medical University,Xuzhou,Jiangsu 221004,China)

机构地区：[1]徐州医科大学医学技术学院,江苏徐州221004

出　　处：《徐州医科大学学报》2025年第2期112-115,共4页Journal of Xuzhou Medical University

基　　金：江苏省高校自然科学基金(23KJB310025);江苏省大学生创新创业训练计划项目(202010313043Y)。

摘　　要：目的检测动脉粥样硬化(AS)患者血清中微小RNA-217(miR-217)的水平,评估其作为AS非侵入性辅助诊断分子标志物的临床价值。方法选取于徐州医科大学附属医院诊治的63例AS患者(病例组),同时选取年龄、性别匹配的22例健康人群(对照组),收集血清标本。采用实时荧光定量PCR检测2组血清miR-217的水平。采用logistic回归分析血清miR-217、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)与AS的相关性。通过受试者操作特征(ROC)曲线分析评估miR-217对AS的诊断效能。结果与对照组相比,病例组HDL-C显著下降,TG显著升高,血清miR-217的表达水平明显上调,差异有统计学意义(P<0.01)。logistic回归分析显示,miR-217显著影响AS的发生(OR=1.51,95%CI:1.045~2.182,P=0.028)。ROC曲线分析显示,当miR-217的截断值为8.851时,其筛查AS患者与健康人群的ROC曲线下面积(AUC)为0.931(95%CI:0.871~0.991),敏感度为0.70,特异度为1。血清miR-217诊断AS的AUC高于血清TG和HDL-C,且miR-217与HDL-C、TG联合诊断AS时的AUC最高。结论AS患者血清miR-217水平较健康人显著升高,是AS的潜在辅助诊断分子标志物。Objective To detect the levels of serum microRNA-217(miR-217)in atherosclerosis(AS)patients and evaluate its clinical value as a non-invasive auxiliary diagnostic molecular marker for AS.Methods A total of 63 AS patients(case group)who were diagnosed and treated at the Affiliated Hospital of Xuzhou Medical University were selected.Meanwhile,22 age-and gender-matched healthy subjects(control group)were included.Their serum samples were collected for detecting serum miR-217 levels by quantitative real-time PCR.Logistic regression analysis was performed to investigate the correlation between serum miR-217,HDL-C,TG,and AS.Receiver operating characteristic(ROC)curves were plotted to assess the diagnostic efficiency of miR-217 for AS.Results Compared with the control group,the case group showed a significant decrease in HDL-C and a significant increase in TG,while serum miR-217 expression was significantly upregulated(P<0.01).Logistic regression analysis indicated that miR-217 significantly affected the occurrence of AS(OR=1.51,95%CI:1.045-2.182,P=0.028).ROC curve analysis showed that when the cutoff value for miR-217 was 8.851,the area under the curve(AUC)for screening AS patients from healthy individuals was 0.931(95%CI:0.871-0.991),with a sensitivity of 0.70 and specificity of 1.The AUC of serum miR-217 for diagnosing AS was higher than that of serum TG and HDL-C,and the AUC for the combined diagnosis of AS using miR-217,HDL-C,and TG was the highest.Conclusions AS patients present significantly higher levels of serum miR-217 than healthy individuals,and miR-217 is a potential auxiliary diagnostic molecular marker for AS.

关 键 词：动脉粥样硬化 微小RNA-217 诊断标志物 ROC分析 临床价值 

分 类 号：R446.1[医药卫生—诊断学]