CRNDE在宫颈癌中的表达及其临床意义  

CRNDE expression in cervical cancer and its clinical significance

作　　者：李怡娟 经莉 裴婉秋 LI Yijuan;JING Li;PEI Wanqiu(Graduate School,Xuzhou Medical University,Xuzhou,Jiangsu 221004,China;Department of Gynecology,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu 221002)

机构地区：[1]徐州医科大学研究生院,江苏徐州221004 [2]徐州医科大学附属医院妇科,江苏徐州221002

出　　处：《徐州医科大学学报》2025年第2期134-139,共6页Journal of Xuzhou Medical University

基　　金：江苏省研究生科研与实践创新计划项目(SJCX_1537)。

摘　　要：目的探讨结直肠癌差异表达基因(CRNDE)在宫颈癌中的表达及其与患者临床特征和预后的关系。方法通过在线数据库分析宫颈癌组织和正常组织中CRNDE的表达差异,并利用实时荧光定量PCR检测临床组织样品以验证生物信息分析结果。进一步分析CRNED的表达水平与宫颈癌患者临床特征的关系。采用单因素和多因素Cox回归分析评估CRNDE表达与宫颈癌患者预后的关系。此外,采用Western blot法检测CRNDE沉默后Hela细胞中ERK、MEK、p-ERK、p-MEK蛋白的表达变化。结果CRNDE在宫颈癌组织中的表达显著上调(P<0.05)。CRNDE的表达水平与宫颈癌的FIGO分期、组织学分级、N分期、M分期显著相关(P<0.05)。高表达CRNDE的宫颈癌患者其生存期明显短于低表达患者(P<0.05)。FIGO分期、组织学分级、T分期、N分期、M分期及CRNDE的表达水平是宫颈癌患者总生存期的独立预测因子。CRNDE沉默后,Hela细胞中p-ERK和p-MEK的表达水平显著下调(P<0.05)。结论CRNDE在宫颈癌组织中的高表达与宫颈癌患者较差的预后相关。CRNDE可能通过调控MAPK/ERK通路,成为宫颈癌的潜在诊断和治疗靶点。Objective To investigate the expression of the colorectal cancer differentially expressed(CRNDE)gene in cervical cancer and its relationship with clinical features and prognosis of patients.Methods The expression differences of CRNDE between cervical cancer tissues and normal tissues were analyzed using online databases.The results were verified with clinical tissue samples using real-time quantitative fluorescence PCR.The relationship between CRNDE expression and clinical features of cervical cancer patients was further analyzed.Univariate and multivariate Cox regression analyses were used to assess the relationship between CRNDE expression and prognosis of cervical cancer patients.Additionally,Western blot was performed to detect changes in the expression of ERK,MEK,p-ERK,and p-MEK proteins in Hela cells after CRNDE silencing.Results CRNDE expression was significantly upregulated in cervical cancer tissues(P<0.05).The expression of CRNDE was significantly associated with the FIGO stage,histological grade,N stage,and M stage of cervical cancer(P<0.05).Patients with high CRNDE expression had a significantly shorter survival time than those with low CRNDE expression(P<0.05).The FIGO stage,histological grade,T stage,N stage,M stage,and CRNDE expression level were independent prognostic factors for the overall survival of cervical cancer patients.After CRNDE silencing,the levels of p-ERK and p-MEK were significantly downregulated in Hela cells(P<0.05).Conclusions High expression of CRNDE in cervical cancer tissues is associated with poor prognosis in cervical cancer patients.CRNDE may serve as a potential diagnostic and therapeutic target for cervical cancer by modulating the MAPK/ERK signaling pathway.

关 键 词：宫颈癌 TCGA数据库 结直肠癌差异表达基因 MAPK/ERK信号通路 

分 类 号：R711.74[医药卫生—妇产科学]