Durotaxis and Topotaxis Orchestrated Guidance on Cell Migration in 3D Printed Scaffold/Hydrogel Composite  

作　　者：Baichuan Deng Zhiwei Huang Xianglin Zhang Kai Ren Wen Feng Lu Jerry Ying Hsi Fuh Xinghuang Liu Tao Bai Bin Wu 

机构地区：[1]State Key Laboratory of Materials Processing and Die&Mould Technology,School of Materials Science and Engineering,Huazhong University of Science and Technology,Wuhan,430074,China [2]State Key Laboratory of Fluid Power and Mechatronic Systems,School of Mechanical Engineering,Zhejiang University,Hangzhou,310027,China [3]Key Laboratory of Advanced Manufacturing Technology of Zhejiang Province,School of Mechanical Engineering,Zhejiang University,Hangzhou,310027,China [4]Department of Mechanical Engineering,National University of Singapore,Singapore,117575,Singapore [5]Division of Gastroenterology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China

出　　处：《Additive Manufacturing Frontiers》2024年第2期224-231,共8页增材制造前沿(英文)

基　　金：supported by National Natural Science Foundation of China(Grant.No.52305558);Hubei Provincial Natural Science Foun-dation of China(Grant.Nos.2023AFB141,2023AFB807);Key Research and Development Plan of Zhejiang Province(Grant.No.2023C01169);Ezhou Industrial Technology Research Institute,Huazhong University of Science and Technology(Customizable Multi-nozzle 3D Bio-printing Technology&Equipment Project).

摘　　要：Printing three-dimensional(3D)scaffolds with suitable mechanical cues is an effective strategy for guiding tissue regeneration by inducing cell migration and growth.Nevertheless,many studies considered only one type of cue for 3D tissue engineering scaffold fabrication,such as topological cues,which is insufficient.To realize durotaxis-and topotaxis-orchestrated guidance on cell migration,a 3D printed scaffold/hydrogel composite was fabricated in this study.The porous scaffold provided a topological cue(topotaxis),and the combined hydrogel provided a compliance cue(durotaxis).The results indicated that the thin fibers of the scaffold induced cell migration,and the larger pore size and directed fiber number of the scaffold led to more uniform cell orientation(topotaxis).Furthermore,when collagen was cured to cover the scaffold to result in a compliance change,the cells in the collagen still sensed the scaffold topological cue and migrated along it(durotaxis).Collagen also provides a living space and nutrition for cells,thereby significantly increasing their number.The effects of durotaxis and topotaxis synthesis provide a promising solution for tissue engineering scaffold applications.

关 键 词：Tissue engineering scaffold Topological cue Compliance cue Cell migration 

分 类 号：R31[医药卫生—基础医学]