玉米KCS家族的全基因组鉴定与胁迫响应分析  

Genome-wide identification and stress response analysis of maize KCS family

作　　者：皮博艺 聂治 杜文平[1] 余桂容[1] 甘雨 宋军[1] PI Bo-yi;NIE Zhi;DU Wen-ping;YU Gui-rong;GAN Yu;SONG Jun(Institute of Biotechnology and Nuclear Technology,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China)

机构地区：[1]四川省农业科学院生物技术核技术研究所,成都610066

出　　处：《西南农业学报》2025年第1期1-11,共11页Southwest China Journal of Agricultural Sciences

基　　金：四川省农业科学院高层次人才引进培养专项(NKYRCZX2024023);“十四五”四川省农作物及畜禽育种攻关项目(2021YFYZ0017);国防科工局核能开发科研项目;四川省科技计划项目(2021YFY0027)。

摘　　要：【目的】鉴定玉米β-酮酰辅酶A合成酶(KCS)家族基因,并对其进行生物信息学和胁迫应答分析,为玉米的遗传改良提供优异的基因参考。【方法】根据拟南芥KCS家族序列,在玉米基因组中筛选鉴定KCS家族成员,采用生物信息学技术对ZmKCS家族成员进行聚类分析、染色体定位、倍增基因分析、基因与蛋白结构分析,并进一步预测KCS家族基因上游顺式作用元件的类型和数目,结合干旱和盐胁迫下ZmKCS家族的表达水平,鉴定兼具抗旱抗盐的优异基因资源。【结果】在玉米基因组数据库中鉴定出39个ZmKCS蛋白,分布在玉米基因组的9条染色体上,聚类于同一亚族的基因具有相似的基因结构和蛋白结构,进化相对保守,同时在玉米KCS家族中发现基因复制现象,揭示KCS家族基因之间可能存在功能冗余;ZmKCS基因上游存在着大量与干旱胁迫应激相关的顺式作用元件,表明ZmKCS基因可能在干旱胁迫等非生物胁迫中发挥重要功能,ζ亚族中ZmKCS1、ZmKCS7、ZmKCS8和ZmKCS10经PEG和NaCl诱导后上调倍数较高,这几个基因可能更具有耐干旱和盐胁迫的潜力。【结论】玉米KCS家族基因具有抗旱抗盐的潜力,通过生物信息学分析以及PEG和NaCl诱导下的玉米KCS基因的表达模式,鉴定出4个最具潜力的基因资源可用于玉米耐旱耐盐品种的遗传改良。【Objective】The article aimed to identify theβ-Ketoacyl-CoA synthase(KCS)family genes in maize genome.Bioinformatics and stress response analysis were carried out in order to provide excellent gene reference for the genetic improvement of maize.【Method】The KCS family members were screened and identified in maize genome according to Arabidopsis KCS family sequence,and the ZmKCS family members were analyzed by cluster analysis,chromosome localization,duplication gene analysis,gene and protein structure analysis by bioinformatics methods.Further,the types and numbers of upstream cis-acting elements of KCS family genes were predicted,combining the expression levels of ZmKCS family under drought and salt stress to identify excellent gene resources with both drought and salt resistance.【Result】In maize genome database,39 ZmKCS proteins were identified,distributed on 9 chromosomes of maize genome.Genes clustered in the same subfamily had similar gene structure and protein structure,and their evolution was relatively conservative.At the same time,gene duplication events were found in KCS family of maize,revealing that there may be functional redundancy among KCS family genes.There were a large number of cis-acting elements related to drought and stress in the upstream of ZmKCS gene,indicating that ZmKCS gene may play an important role in abiotic stress such as drought stress.ZmKCS1,ZmKCS7,ZmKCS8 and ZmKCS10 in theζsubfamily had a higher up-regulation after induction of PEG and NaCl.The result suggested that these genes may be more resistant to drought and salt stress.【Conclusion】ZmKCS family genes have the potential of drought and salt tolerance.Through bioinformatics analysis and the expression patterns of maize KCS genes under PEG and NaCl treatment,four most potential gene resources were identified for genetic improvement of drought and salt tolerance maize varieties.

关 键 词：玉米 β-酮酰辅酶A合成酶 生物信息学 胁迫响应 

分 类 号：S513[农业科学—作物学]