丛枝菌根真菌群落对不同人工植被修复及混播燕麦的响应机制研究  

Responses of arbuscular mycorrhizal fungal communities to various artificial vegetation restoration practices and oat intercropping

作　　者：刘哲荣 杨逸 白文科 秦明森 LIU Zhe-rong;YANG Yi;BAI Wen-ke;QIN Ming-sen(Forestry and Grassland Bureau of Shiqu County,Ganzi,Sichuan 627350,China;Key Laboratory of Southwest China Wildlife Resources Conservation(Ministry of Education),China West Normal University,Nanchong,Sichuan 637001,China)

机构地区：[1]石渠县林业和草原局,四川甘孜627350 [2]西华师范大学西南野生动植物资源保护教育部重点实验室,四川南充637001

出　　处：《西南农业学报》2025年第1期171-181,共11页Southwest China Journal of Agricultural Sciences

基　　金：石渠县优质牧草品种选育、栽培技术、储藏加工等成套技术研究;西南野生动植物资源保护教育部重点实验室开放基金项目(XNYB21-04);四川省自然科学基金项目(2023NSFSC1223)。

摘　　要：【目的】丛枝菌根真菌(AMF)作为植物根部共生微生物,能够提高植物生长和土壤稳定性。研究AMF多样性和群落组成对不同人工植被种类修复与混播燕麦的响应特征,有助于筛选高效修复植被、完善生态修复策略。【方法】设置7种牧草播种和混播燕麦,共14种人工播种修复样地。经过3年自然恢复,测定分析土壤理化、植物生长和群落以及AMF群落。【结果】播种植物种类对植物生长和群落结构有显著影响,其中单播植物种类的效应大于混播燕麦。植物群落特征和多样性指标中,仅植物地上生物量和盖度在红豆草和燕麦混播处理下更高。播种植物种类显著影响土壤pH、速效氮、总磷,而混播燕麦对土壤性质影响较小。播种植物种类对AMF的谱系多样性和丰富度,以及群落组成有显著影响,而混播燕麦对AMF群落的影响不显著。土壤速效氮对AMF的亲缘关系指数的影响较强,而土壤pH对AMF的谱系多样性和丰富度均有较强影响;同时,土壤pH、总磷和植物氮磷比与AMF群落组成呈显著相关。在人工植被修复中,混播燕麦能影响植物生物量、氮磷含量以及植物群落组成,但单播植物的种类对土壤pH和速效氮,以及植物生长特征和群落组成和多样性均有影响,且对AMF的群落结构和多样性起决定作用;此外,土壤AMF群落组成还受土壤pH、总磷及植物氮磷比的影响。【结论】牧草额外混播燕麦并不能显著改变土壤理化和AMF群落,而某种牧草的播种并不能同时提高土壤营养和AMF多样性,要根据生态修复的需要,选择适宜的牧草种类。【Objective】Arbuscular mycorrhizal fungi(AMF) are symbiotic microorganisms associated with plant roots that can enhance plant growth and soil stability.Investigating AMF communities and diversity under different vegetation types in artificial restoration is crucial for refining ecological restoration theories and selecting effective restorative vegetation.【Method】The present study established 14 artificial sowing restoration plots with 7 grass species and oat intercropping.After 3 years of natural recovery, we measured the soil properties, the growth characteristics and communities of plant, and the soil AMF community.【Result】The study revealed that the sown plant species significantly impacted plant growth and community structure, with single-sown species having a more pronounced effect than oat intercropping.Among the plant community characteristics and diversity indices, only aboveground biomass and plant cover were significantly higher under Onobrychis viciifolia and oat intercropping treatment.The sown plant species significantly influenced soil pH,available nitrogen and total phosphorus, while oat intercropping had minimal impact on soil properties.The sown plant species had a significant effect on AMF phylogenetic diversity and richness, as well as community composition, whereas oat intercropping had a negligible impact on AMF.Soil available nitrogen had a strong explanatory effect on the AMF nearest taxon index, while soil pH significantly influenced AMF phylogenetic diversity and richness.Soil pH,total phosphorus, and plant nitrogen-to-phosphorus ratio also played important roles in the changes in AMF communities.In artificial vegetation restoration, intercropping oats could influence plant biomass, nitrogen and phosphorus content, and plant community composition.However, the species of monoculture plants had an impact on soil pH,available nitrogen, and plant growth characteristics, community composition and diversity.Moreover, they played a decisive role in the community structure and diversi

关 键 词：丛枝菌根真菌 人工修复 混播燕麦 牧草 多样性 

分 类 号：S154.3[农业科学—土壤学]