川陕哲罗鲑髓样分化因子88的克隆、表达及免疫功能分析  

Cloning, expression and immune function of myeloid differentiation factor 88 in Sichuan taimen (Hucho bleekeri)

作　　者：陈叶雨 吴晓雲 杨焕超 刘钊 陈彦伶[1] 林珏[1] 李华[1] 文典[2] 王渝栋 谭平[2] CHEN Ye-yu;WU Xiao-yun;YANG Huan-chao;LIU Zhao;CHEN Yan-ling;LIN Jue;LI Hua;WEN Dian;WANG Yu-dong;TAN Ping(Fisheries Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 611731,China;Powerchina Chengdu Engineering Corporation Limited,Chengdu 610072,China)

机构地区：[1]四川省农业科学院水产研究所,成都611731 [2]中国电建成都勘测设计研究院有限公司,成都610072

出　　处：《西南农业学报》2025年第1期182-191,共10页Southwest China Journal of Agricultural Sciences

基　　金：四川省农业科学院原始创新2035项目(YSCX2035-011);四川省财政自主创新专项(2022ZZCX093);四川省淡水鱼创新团队(YSCX2035-011);农业农村部长江流域渔业渔政管理局办公室(cjb2024wzbh-011);珍稀鱼类生物学及人工驯养繁殖研究(CD2C20242330)。

摘　　要：【目的】疾病防控是濒危物种保护管理不可缺少的一个方面,为更深入了解我国一级保护动物川陕哲罗鲑的免疫系统特征,对炎症通路的关键分化因子MyD88(髓样分化因子88)在川陕哲罗鲑中的潜在免疫功能进行研究。【方法】利用川陕哲罗鲑全长转录组数据库获得MyD88基因的cDNA序列,分析其氨基酸结构及进化关系,同时分析其在健康川陕哲罗鲑各组织及LPS和poly(I:C)刺激下的表达模式。【结果】川陕哲罗鲑MyD88具有典型的死亡结构域和TIR结构域。氨基酸序列比较表明,川陕哲罗鲑MyD88与其他脊椎动物具有较高的同源性,其中与鲑科鱼类的同源性最高。系统发育分析表明,川陕哲罗鲑MyD88与鲑科鱼类聚为一支;此外,它们与其他鱼类的同源基因聚在一起,而哺乳动物的MyD88聚集在一个单独的分支上。组织分布分析显示,MyD88在所有组织中广泛表达,其中在头肾、中肾、脾脏和鳃中均高表达。LPS和poly(I:C)刺激后,头肾和脾脏中MyD88的表达显著上调。【结论】研究揭示了MyD88基因在川陕哲罗鲑免疫应答中的潜在作用,为今后川陕哲罗鲑的疾病防治提供理论依据。【Objective】Disease prevention and control is an indispensable aspect in the process of conservation and management of endangered species.In order to better understand the characteristics of immune system of Hucho bleekeri,a first class protected animal in China, the potential immune function of a key differentiation factor of inflammatory pathway MyD88 was analyzed in the study.【Method】The cDNA sequence of MyD88 was obtained by using the full-length transcriptome database, and the amino acid structure and evolutionary relationship of MyD88 were analyzed.Meanwhile, the expression profiles of MyD88 in different tissues and the expression patterns upon LPS and poly(I:C) stimulation were analyzed.【Result】The MyD88 of H.bleekeri had death domain and TIR domain.Amino acid sequence comparison revealed that MyD88 of H.bleekeri had high homology with other vertebrates, among which homology with salmonid fish was the highest.Phylogenetic analysis showed that H.bleekeri MyD88 clustered with salmonid fish.Moreover, H.bleekeri MyD88 clustered with its homologous genes of other fish, whereas mammalian MyD88 clustered into a separate branch.Tissue distribution analysis revealed MyD88 was widely expressed in all tested tissues, with high expression detected in the head kidney, trunk kidney, spleen and gill.After LPS and poly(I:C) stimulation, the expression levels of MyD88 in the head kidney and spleen of H.bleekeri were significantly up-regulated.【Conclusion】The study reveals the potential role of MyD88 in the immune response of H.bleekeri and provides a reference for its future disease control management.

关 键 词：髓样分化因子88 川陕哲罗鲑 组织分布 免疫反应 

分 类 号：S917.4[农业科学—水产科学]