截短型水痘带状疱疹病毒gE融合分子内佐剂的原核表达及其免疫原性  

Prokaryotic expression and immunogenicity of truncated varicella zoster virus gE fused with intramolecular adjuvant

作　　者：刘晓菲 孙苓苓 LIU Xiaofei;SUN Lingling(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2025年第3期290-294,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的原核表达水痘带状疱疹病毒gE融合分子内佐剂的融合蛋白,并评价其免疫原性。方法以pET28-a质粒为载体,构建水痘带状疱疹病毒蛋白E(glycoprotein E,gE)融合破伤风毒素(tetanus toxoid,TT)通用表位P2基因,并将其转化至大肠杆菌中,经IPTG诱导表达,亲和色谱纯化,获得纯度较高的gE-TT蛋白。将gE-TT蛋白免疫小鼠,检测小鼠的血清特异性IgG抗体滴度,评价其免疫原性。结果构建的重组大肠杆菌工程菌经IPTG诱导能稳定的表达gE-TT蛋白,经分离纯化后,获得分子量约为62 kD的gE-TT蛋白,并能与anti-VZV gE抗体特异性结合;ELISA检测显示小鼠血清产生了较强的gE-TT特异性IgG抗体。结论本研究已在大肠杆菌系统中成功表达了重组gE-TT蛋白,该蛋白可有效引起小鼠的体液免疫反应,为水痘带状疱疹病毒的实验室研究和疫苗制备奠定基础。Objective The fusion protein of the intramolecular adjuvant of varicella zoster virus gE fusion was expressed in prokaryotic cells and its immunogenicity was evaluated.Methods The varicella zoster virus gE protein fused with tetanus toxin(TT)universal epitope P2 was constructed using the pET28-a plasmid as a vector and transformed into Escherichia coli,where it was induced to be expressed by IPTG and purified by affinity chromatography to obtain the gE-TT protein with high purity.The gE-TT protein was immunized to mice,and the serum specific IgG antibody titer of mice was detected to evaluate its immunogenic effect.Results The constructed recombinant Escherichia coli engineering bacteria induced by IPTG could stably express gE-TT protein.After being isolated and purified,gE-TT protein with molecular weights of about 62 kD was obtained,and it could be specifically bound to the murine anti VZV gE glycoprotein monoclonal antibody;ELISA assay showed that the mouse serum produced a strong gE-TT-specific IgG antibody.Conclusion In this study,the gE-TT protein has been successfully expressed in the E.coli system,which can effectively elicit humoral immune response in mice,and lays the foundation for laboratory research and vaccine preparation for varicella zoster virus.

关 键 词：水痘带状疱疹病毒 gE-TT抗原 原核表达 免疫原性 

分 类 号：R96[医药卫生—药理学]