梨亚科5个属S-RNase基因的序列特征及进化分析  

Sequence characterization and evolutionary analysis of S-RNase gene among five genera Pomoideae

作　　者：梁文杰[1] 谢志亮[1] 乌云塔娜[2] LIANG Wenjie;XIE Zhiliang;Wuyun Tana(Wenzhou Vocational College of Science and Technology,Wenzhou 325006,Zhejiang,China;Research Institute of Non-Timber Forestry of Chinese Academy of Forestry,Zhengzhou 450003,Henan,China)

机构地区：[1]温州科技职业学院,浙江温州325006 [2]中国林业科学研究院经济林研究所,郑州450003

出　　处：《果树学报》2025年第3期462-475,共14页Journal of Fruit Science

基　　金：国家自然科学基金项目(31000309);温州市自然资源和规划局专项(2023)。

摘　　要：【目的】分析梨亚科5个属S-RNase基因序列特征及其进化规律。【方法】收集GenBank数据库中5个属S-RNase基因CDS全长、大于330 bp的外显子片段以及内含子序列,剔除重复序列后,利用MEGA11软件对其进行序列和多态性分析,并利用多序列比对结果构建系统进化树;计算S-RNase基因RSCU值,以欧式平方距离作为基因间进化距离进行聚类分析。利用MEGA11软件计算梨亚科5个属S-RNase基因序列碱基组成及密码子使用偏好。【结果】GenBank数据库中剔除重复序列后,共收录梨亚科5个属S-RNase基因CDS全长序列90条,长度范围为678~711 bp;大于330 bp的外显子片段序列有140条。梨亚科5个属S-RNase基因序列各区域分析表明:C2-HV、C4-C5、C5-和HV区存在较多的共性特征;编码区、内含子、密码子使用偏好聚类的进化树均没有明显种和属的界限。S-RNase基因3个位置的碱基含量均呈现A+T大于C+G,HV区3个位置CG分布较为一致。【结论】梨亚科5个属S-RNase基因除了HV区,C2-HV、C4-C5和C5-也具备参与S位点识别的可能性。同时,梨亚科5个属S-RNase基因的分化早于5个属的分化时间且SRNase基因密码子存在一定的偏好。【Objective】In order to avoid self-pollination,self-incompatibility is a common phenomenon in 5 genera in Pyridae fruit trees during evolution.In order to understand the latest isolation and identification of S-RNase gene in pear incompatibility,we analyzed its sequence characteristics and evolutionary rules.【Methods】The keywords Malus S-RNase complete cds,Pyrus S-RNase complete cds,Cydonia S-RNase complete cds,Crataegus S-RNase complete cds and Eriobotrya S-RNase complete cds were searched for the full-length CDS sequence of pear S-RNase gene in the GenBank database of NCBI.And the Blast tool was use to search and supplement.After the initial identification of the searched sequences,the sequence analysis was performed using VectorNTI11.5.3 software,and the duplicate sequences were manually corrected and eliminated.The Find Best DNA/Protein Models program of MEGA11 software was used to find out the optimal model suitable for the sequence of 5 genera of Pyridae S-RNase gene,and the corresponding model and algorithm were used to calculate the differentiation between the sequences.Tajima's Test of Neutrality under Selection was used to calculate genetic polymorphisms,and the sequences of signal peptide,C1-C5,HV region and other relevant regions were analyzed respectively.The nucleotide composition and Compute codon usage bias programs of MEGA11 software were used to calculate the base composition and relative synonymous of the SRNase gene sequence of 5 genera of Pyridae codon usage value.The ClustalW program of MEGA11 software was used to compare the CDS full-length sequence of 5 genera in Pyridae S-RNase gene.The phylogenetic tree of gene coding region was constructed by NJ in distance method,and the reliability test was performed 1000 times by Bootsrap.SPSS22 was used to cluster the S-RNase gene RSCU of 5 genera of Pyridae using Euclidean square distance as inter-gene evolutionary distance.【Results】At present,120 CDS full-length sequences of 5 genera in Pyridae S-RNase gene were included in Gen-Bank dat

关 键 词：梨亚科 雌蕊S基因 序列特征 密码使用偏好 进化分析 

分 类 号：S661.2[农业科学—果树学]