不同生长期棉花叶片中类黄酮代谢物的变化与分布  

Changes and distribution of flavonoid metabolites in cotton leaves at different growth stages

作　　者：刘璐瑶 曹倩文 马晓鸽 秦兆隆 刘梦格 汤梦琪 钟超敏 商海红 陈迪[1] 屈凌波[1] 徐霞[1] Liu Luyao;Cao Qianwen;Ma Xiaoge;Qin Zhaolong;Liu Mengge;Tang Mengqi;Zhong Chaomin;Shang Haihong;Chen Di;Qu Lingbo;Xu Xia(Zhengzhou University/State Key Laboratory of Cotton Bio-breeding and Integrated Utilization,Zhengzhou 450001,China)

机构地区：[1]郑州大学/棉花生物育种与综合利用全国重点实验室,郑州450001

出　　处：《棉花学报》2025年第1期1-12,共12页Cotton Science

基　　金：棉花生物育种与综合利用全国重点实验室开放课题基金(CBIU2023ZZ13;CBIU2023ZZ03)。

摘　　要：【目的】探究不同生长发育时期棉花叶片中类黄酮次生代谢物的动态变化过程。【方法】取陆地棉品种sGK156苗期、盛花期和吐絮期的叶片,利用超高效液相色谱-串联质谱对棉花叶片中的差异代谢物进行分析,并对类黄酮物质丰度进行检测。【结果】3个不同时期的棉花叶片差异化合物主要富集在黄酮和黄酮醇的生物合成代谢通路;苗期山柰酚-3-O-阿拉伯糖苷、柚皮素的含量显著高于盛花期和吐絮期,盛花期紫云英苷、银椴苷、槲皮素等16种类黄酮化合物的含量显著高于苗期和吐絮期,吐絮期表儿茶素、山柰酚-3-O-芸香糖苷、山柰酚-3-O-巢菜糖苷、原花青素B2、秦皮苷这5种化合物的含量显著高于苗期和盛花期。【结论】本研究分析类黄酮次生代谢物在棉花不同生长发育时期的动态变化过程,发现了在不同生长时期棉花叶片中优势表达的类黄酮代谢物,为进一步研究与利用棉花叶片中类黄酮代谢产物和选育优良棉花品种提供理论基础。[Objective]This study aims to investigate the dynamic changes of flavonoids in cotton leaves at different growth and development periods.[Methods]Cotton leaves of sGK156 at seedling stage,flourishing flowering stage,and boll opening stage were used as study materials,and the differential metabolites were analysed and flavonoids abundance was detected by ultra-high-performance liquid chromatography-tandem mass spectrometry.[Results]Differential compounds of cotton leaves at three different periods were mainly enriched in the biosynthesis of flavone and flavonol,and the biosynthesis of flavonoids.Compared with those at the flourishing flowering stage and boll opening stage,kaempferol-3-O-arabinopyranoside and naringenin in cotton leaves were significantly higher at the seedling stage,and the contents of 16 flavonoids such as astragalin,tiliroside,and quercetin in cotton leaves at flourishing flowering stage were significantly higher than those at seedling stage and boll opening stage,and the contents of 5 compounds of epicatechin,kaempferol-3-O-rutinoside,kaempferol-3-O-vicianoside,procyanidin B2,and fraxin were significantly higher at the boll opening stage compared with seedling stage and flourishing flowering stage.[Conclusion]This study further analyses the dynamic changes of flavonoid secondary metabolites in cotton leaves during different growth periods,and discover the dominantly expressed flavonoid metabolites in cotton leaves during different growth periods.It provides a theoretical basis for the further study and utilization of flavonoid metabolites in cotton leaves and the selection and breeding of excellent cotton varieties.

关 键 词：棉花 叶片 类黄酮化合物 代谢组学 

分 类 号：R28[医药卫生—中药学]