瓜蒌薤白半夏汤调控心脏FGF21因子干预冠心病痰浊壅塞证的机制研究  

Mechanism of Gualou Xiebai Banxia Decoction(瓜蒌薤白半夏汤)Regulating Cardiac FGF21 in Intervention of Coronary Heart Disease with Pattern of Turbid Phlegm Obstruction

作　　者：官帅 王海宇 孙滕 张志伟 赵启韬[1] GUAN Shuai;WANG Haiyu;SUN Teng;ZHANG Zhiwei;ZHAO Qitao(Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《山东中医药大学学报》2025年第2期215-223,共9页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81573852);山东省自然科学基金项目(编号:ZR2021LZY042)。

摘　　要：目的:探讨瓜蒌薤白半夏汤治疗冠心病痰浊壅塞证(CHD-TZ)的机制。方法:70只SD大鼠适应性饲养1周。随机选取10只作为对照组,10只行冠状动脉左前降支结扎(LAD)手术作为普通冠心病(CHD)组。50只大鼠灌胃高脂乳剂HEF,构建高脂血症模型,造模成功后选取10只作为高血脂组;其余40只分为4组,分别灌胃生理盐水及不同剂量瓜蒌薤白半夏汤后行LAD手术,构建CHD-TZ模型。剔除造模失败大鼠,最终确定为对照组、CHD组、高血脂组、CHD-TZ组及瓜蒌薤白半夏汤高、中、低剂量(GXBD-H、GXBD-M、GXBDL)组,各8只。2,3,5-三苯基氯化四氮唑(TTC)染色检测心肌梗死面积,酶联免疫吸附(ELISA)法检测血浆肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、血脂四项[高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDLC)、总甘油三酯(TG)、总胆固醇(TC)]及成纤维细胞生长因子21(FGF21)的水平。蛋白免疫印迹(WB)法检测心肌组织蛋白表达或磷酸化水平。结果:CHD-TZ组大鼠的心肌梗死率明显高于CHD组大鼠(P<0.01);与CHD-TZ组比较,GXBD-H、GXBD-M、GXBD-L组大鼠的心肌梗死率明显降低,差异均有统计学意义(P<0.01)。CHD组大鼠CK、CK-MB高于对照组,CHD-TZ组大鼠CK、CK-MB明显高于CHD组和GXBD-H、GXBDM、GXBD-L组,差异均有统计学意义(P<0.01)。与对照组比较,高血脂组和CHD-TZ组大鼠血浆TC、TG、LDL-C含量升高,HDL-C含量降低,差异有统计学意义(P<0.01);GXBD-H、GXBD-M、GXBD-L组的TC、TG、LDL-C含量均低于CHD-TZ组,且中剂量组含量最低,差异有统计学意义(P<0.01)。高血脂组大鼠血浆FGF21含量高于对照组,CHD组大鼠血浆FGF21含量高于高血脂组,GXBD-M组大鼠血浆FGF21含量低于CHD-TZ组,差异均有统计学意义(P<0.01)。WB检测结果及其相对定量数据显示,大鼠心脏FGF21蛋白表达水平从低到高分别为对照组、高血脂组、CHD组、CHD-TZ组,GXBD-M组大鼠心脏FGF21表达可回落到低水平,差异均有�Objective:To investigate the mechanism of Gualou Xiebai Banxia Decoction(瓜蒌薤白半夏汤)in the treatment of coronary heart disease with pattern of turbid phlegm obstruction(CHD-TZ).Methods:Seventy SD rats were fed adaptively for 1 week.Ten rats were randomly selected as the control group,and 10 rats underwent left anterior descending coronary artery ligation(LAD)as the common coronary heart disease(CHD)group.Fifty rats were given high fat emulsion(HEF)to establish hyperlipidemia model,and ten rats were selected as hyperlipidemia group.The other forty rats were divided into 4 groups,and LAD was performed after gavage of normal saline and different doses of Gualou Xiebai Banxia Decoction,respectively.The model of blockage of phlegm and turbidity in coronary heart disease was established.The rats with failed modeling were excluded,and were finally identified as control group,CHD group,hyperlipidemia group,CHD-TZ group and Gualou Xiebai Banxia Decoction high-,medium-and low-dose(GXBD-H,GXBD-M,GXBD-L)groups,with 8 rats in each group.Myocardial infarction size was measured by 2,3,5-triphenyltetrazolium chloride(TTC)staining.Enzyme-linked immunosorbent assay(ELISA)was used to detect levels of plasma creatine kinase(CK),creatine kinase isoenzyme(CK-MB),four blood lipids[high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),total triglyceride(TG),total cholesterol(TC)]and fibroblast growth factor 21(FGF21).Western blot(WB)was used to detect the protein expression or phosphorylation level of myocardial tissue.Results:The myocardial infarction rate in CHD-TZ group was significantly higher than that in CHD group(P<0.01).Compared with the CHD-TZ group,the myocardial infarction rates of the GXBD-H,GXBD-M,and GXBD-L groups were significantly decrease,and the differences were statistically significant(P<0.01).The levels of CK and CK-MB in the CHD group were significantly higher than those in the control group,and the levels of CK and CK-MB in the CHD-TZ group were significantly higher than

关 键 词：瓜蒌薤白半夏汤 冠心病痰浊壅塞证 心肌缺血 高血脂 成纤维细胞生长因子21 

分 类 号：R259[医药卫生—中西医结合]