Dietary inositol supplementation improves meat quality by modulating amino acid metabolism and gut microbiota composition of finishing pigs  

作　　者：Enfa Yan Haijun Sun Linjuan He Boyang Wan Ming Shen Qiyuan Miao Jingdong Yin Xin Zhang 

机构地区：[1]State Key Laboratory of Animal Nutrition and Feeding,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China [2]Frontiers Science Center for Molecular Design Breeding(MOE),Beijing 100193,China

出　　处：《Animal Nutrition》2024年第4期180-191,共12页动物营养(英文版)

基　　金：supported by the National Natural Science Foundation of China(grant number 32102555);the National Key Research and Development Program of China(grant number 2023YFD1301301).

摘　　要：Intramuscular fat(IMF)content influences various meat quality traits,including tenderness,flavor,juiciness and nutritional value.This study aimed to investigate the effects of dietary inositol supplementation on meat quality,metabolic profiles,and gut microbiota composition of finishing pigs.A total of 144 finishing pigs(initial body weight 70.41±0.78 kg)were randomly divided into control,0.075%,0.15%,and 0.3%inositol groups.The data showed that inositol increased backfat thickness at the 6th to 7th rib and 10th rib,IMF content,and improved tenderness(P≤0.05,n=8).Paralleling an increase in fat deposition,0.3%inositol also increased the protein level of PPARg in the subcutaneous fat(P≤0.05)and longissimus thoracis(LT)muscle(P=0.062).Inositol elevated the content of amino acids in LT muscle and enhanced amino acid metabolism of finishing pigs,including lysine degradation,tyrosine metabolism,and arginine and proline metabolism.The 16S ribosomal RNA(rRNA)sequencing showed that 0.3%inositol supplementation altered the profiles of microbes in the colon,particularly decreasing the abundance of Firmicutes(P<0.01)and increasing the abundance of Bacteroidota(P<0.05).Correlation analysis showed that differential microbes had strong correlation with differential metabolites in serum,including amino acids.In conclusion,this study demonstrated that dietary inositol supplementation could effectively improve IMF content and tenderness of pork,enhance amino acid metabolism,and regulate gut microbiota composition of finishing pigs.

关 键 词：Dietary inositol supplementation Finishing pig Gut microbiota Intramuscular fat content Metabolic profiles Meat quality 

分 类 号：O62[理学—有机化学]