原产地与引种地大花黄牡丹根系和根际土壤的AMF群落差异  

Differences in Arbuscular Mycorrhizal Fungi Community in the Roots and Rhizosphere Soil of Paeonia ludlowii Between Original Site and Introduction Site

作　　者：李超楠 李璐 高丹蕾 乔红雍 袁涛 LI Chaonan;LI Lu;GAO Danlei;QIAO Hongyong;YUAN Tao(Engineering Research Center of Landscape Environment of Ministry of Education,Beijing Laboratory of Urban and Rural Ecological Environment,National Engineering Research Center for Floriculture,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;Luoyang tianyou Garden Limited Company,Luoyang,Henan 471000,China)

机构地区：[1]北京林业大学园林学院,花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,园林环境教育部工程研究中心,北京100083 [2]洛阳天囿园林发展有限公司,河南洛阳471000

出　　处：《园艺学报》2025年第2期467-480,共14页Acta Horticulturae Sinica

基　　金：国家林草局行业标准项目(2018-LY-054);北京园林绿化增彩延绿科技创新工程项目(2019-KJC-02-10)。

摘　　要：以大花黄牡丹(Paeonia ludlowii)原产地西藏自治区林芝市及其引种地河南省栾川县的大花黄牡丹根系和根际土壤为研究对象,在其生长发育的3个关键时期——花期、果期和秋色叶期,利用高通量测序技术,探究丛枝菌根真菌(arbuscular mycorrhiza fungi,AMF)群落结构及多样性差异。结果表明,在3个生长期原产地大花黄牡丹根系AMF群落ObservedOTU均高于引种地,花期根际土壤AMF群落Observed OTU和Chao1指数显著高于引种地,但秋色叶期,引种地Observed OTU显著高于原产地;主成分分析结果显示,两地大花黄牡丹根系AMF群落在花期存在显著差异,根际土壤AMF群落结构在3个生长期均有显著差异,LEfSe分析进一步说明了两地大花黄牡丹根系和根际土壤AMF类群具有显著差异。根孢囊霉属(Rhizophagus)、球囊霉属(Glomus)和多样孢囊霉属(Diversispora)是大花黄牡丹根际AMF群落中的优势种群,在两地大花黄牡丹根系及根际土壤中均有分布,但相对丰度存在明显差异。引种栽培地的土壤环境使大花黄牡丹根系中根孢囊霉属和球囊霉属的相对丰度降低,但多样孢囊霉属和近明球囊霉属(Claroideoglomus)的相对丰度增加。巨孢囊霉属(Gigaspora)仅存在于原产地土壤中,和平囊霉属(Pacispora)仅存在于引种地土壤中。土壤理化性质与AMF群落Mantel分析结果表明,全氮含量是影响引种地大花黄牡丹根系AMF群落的主要因子,土壤速效钾含量与原产地大花黄牡丹根系AMF群落、引种地土壤AMF群落显著相关,有效磷含量与原产地土壤AMF群落极显著相关。The roots and rhizosphere soil of Paeonia ludlowii grown in Nyingchi City,Tibet Autonomous Region(the original site),and Luanchuan County,Henan Province(the introduction site),were used as materials.Based on the high-throughput sequencing technology,this study investigated the differences in the community structure and diversity of the arbuscular mycorrhizal fungi(AMF)in the roots and rhizosphere soil of P.ludlowii from two locations,taking into account the three critical periods of P.ludlowii growth and development-flowering stage,fruit stage and autumn foliage stage.The results showed that the Observed OTU of the AMF community in the roots of P.ludlowii from the original site were higher than those from the introduction site in all three growth periods.The Observed OTU and Chao1 index of the AMF community in the rhizosphere soil of P.ludlowii from the original site were significantly higher than those from the introduction site during the flowering period.However,during the autumn foliage period,the Observed OTU of the AMF community in the rhizosphere soil from the introduction site were significantly higher than those from the original site.The results of principal component analysis showed that the AMF community in the roots of P.ludlowii differed significantly during the flowering period,and the soil AMF community structure differed significantly in all three growth periods,the LEfSe analysis further illustrated that the AMF species in the roots and rhizosphere soil of P.ludlowii differed significantly between the two sites.Rhizophagus,Glomus and Diversispora were the dominant species in the AMF communities in the roots and rhizosphere soil of P.ludlowii from the two sites,but their relative abundance differed significantly between the two sites.Due to the soil environment of the introduction site,the relative abundance of Rhizophagus and Glomus in the AMF community in the roots of P.ludlowii decreased,while the relative abundance of Diversispora and Claroideoglomus increased.Gigaspora was present only in th

关 键 词：大花黄牡丹 丛枝菌根真菌 高通量测序 群落结构 

分 类 号：S685.11[农业科学—观赏园艺]