甘肃定西地区辣椒病毒病鉴定及BWYV遗传变异分析  

Identification of Pepper Virus Diseases and Analysis of BWYV Genetic Variation in Dingxi Area of Gansu Province

作　　者：杨文清 王嘉荟 徐秉良 李惠霞 梁巧兰 杨顺义 牛二波 YANG Wenqing;WANG Jiahui;XU Bingliang;LI Huixia;LIANG Qiaolan;YANG Shunyi;NIU Erbo(College of Plant Protection,Gansu Agricultural University,Anning,Gansu 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃安宁730070

出　　处：《园艺学报》2025年第2期491-502,共12页Acta Horticulturae Sinica

基　　金：甘肃农业大学人才启动金项目(GAU-KYQD-2022-27);甘肃农业大学植物病害绿色防控团队建设项目(26000102);国家自然科学基金青年科学基金项目(32302332);甘肃省青年科技基金项目(23JRRA1431)。

摘　　要：利用高通量测序技术和RT-PCR相结合的方法,对甘肃定西地区感染病毒病的辣椒叶片进行病原鉴定。结果显示染病叶片受黄瓜花叶病毒(cucumber mosaic virus,CMV)和甜菜西方黄化病毒(beet western yellows virus,BWYV)的复合侵染,首次在甘肃发现BWYV侵染辣椒。借助特异性引物克隆BWYV的外壳蛋白(coat protein,CP)和运动蛋白(movement protein,MP)基因深入分析BWYV的分子进化和遗传变异,通过序列比对发现其CP基因与其他物种BWYV分离物的核苷酸和氨基酸相似性分别为93.27%~99.51%和90.10%~99.01%,其MP基因的相似性分别为89.20%~99.05%和86.86%~98.86%。此外,系统发育树分析显示BWYV不同分离物可分为4个类群,无明显地理分布特征,寄主植物多样性分析显示具有显著的寄主关联性。Using a method that combines high-throughput sequencing technology with RT-PCR,pathogen identification was conducted on pepper leaves infected with viral diseases in the Dingxi area of Gansu Province.The results indicated that the infected leaves were co-infected with cucumber mosaic virus and sugar beet western yellow virus.Notably,this study marks the first identification of BWYV infecting peppers in Gansu.Specific primers were employed to clone the coat protein and movement protein genes of BWYV,facilitating an in-depth analysis of the molecular evolution and genetic variation of this virus.Sequence comparisons revealed that the nucleotide and amino acid similarities between the coat protein(CP)gene of BWYV and isolates from other species ranged from 93.27%to 99.51%and from 90.10%to 99.01%,respectively.The similarities of the movement protein(MP)genes were found to be between 89.20%and 99.05%for nucleotides and between 86.86%and 98.86%for amino acids.Furthermore,phylogenetic analysis of BWYV demonstrated that different isolates could be categorized into four distinct groups.These groups exhibited no discernible geographical distribution characteristics,while host plant diversity analysis revealed significant correlations with host species.

关 键 词：辣椒 甜菜西方黄化病毒 RT-PCR检测 进化分析 

分 类 号：S641.3[农业科学—蔬菜学]