黄芩苷-栀子苷调节 HMGB1/PI3K/AKT 信号通路减少脑缺血再灌注后继发的肺损伤  

Baicalin-Geniposide modulates the HMGB1/PI3K/AKT signaling pathway to reduce lung injury following cerebral ischemia-reperfusion

作　　者：李晓秋 龙宇 邓婕 马茵 吴媛媛[1] 胡月 贺小方 李楠[1] LI Xiaoqiu;LONG Yu;DENG Jie;MA Yin;WU Yuanyuan;HU Yue;HE Xiaofang;LI Nan(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Sichuan Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,四川成都610075

出　　处：《中药与临床》2025年第1期46-49,66,共5页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省科技厅项目(2022NSFSC1406)。

摘　　要：目的:研究黄芩苷-栀子苷(BG)对脑缺血再灌注后继发肺损伤的干预作用,并探讨其对肺部HMGB1/PI3K/AKT蛋白表达的影响。方法:大鼠分为空白组、脑缺血再灌注模型组、BG低剂量组和BG高剂量组。除空白组外,其余组建立脑缺血再灌注模型。记录体质量,观察肺部情况,检测肺泡灌洗液总蛋白、MDA和SOD水平,使用免疫荧光和Western Blot法检测HMGB1和相关蛋白表达。结果:与空白组比较,模型组大鼠体质量下降,肺组织受损,肺泡灌洗液中总蛋白含量上升,肺组织MDA水平上升、SOD水平下降。而BG高低剂量组(50、25 mg·mL^(-1))能缓解这些变化,且呈现剂量依赖性。免疫荧光和Western Blot结果显示,模型组HMGB1及相关蛋白表达增加,BG给药组表达降低。结论:BG对脑缺血再灌注后继发的肺损伤具有干预作用,可能通过调节抗氧化酶活性、抑制HMGB1/PI3K/AKT通路相关蛋白表达,减轻大鼠脑缺血再灌注后继发肺损伤。Objective:To investigate the intervention effect of Baicalin-Gardenoside(BG)on secondary lung injury following cerebral ischemia-reperfusion and explore its influence on the expression of HMGB1/PI3K/AKT proteins in the lungs.Methods:Rats were divided into blank group,cerebral ischemia-reperfusion model group,low-dose BG group,and high-dose BG group.Except the blank group,the remaining groups all underwent cerebral ischemia-reperfusion modeling.Body weight was recorded,and lung conditions were observed.Levels of total protein,MDA,and SOD in lung lavage fluid were measured.The expression of HMGB1 and related proteins was detected using immunofluorescence and Western Blot methods.Results:Compared with the blank group,rats in the model group showed decreased body weight,lung tissue damage,increased total protein in lung lavage fluid,elevated MDA levels,and decreased SOD levels in lung tissue.However,the BG high and low-dose groups(25 and 50 mg·mL^(-1))alleviated these changes,showing a dosedependent relationship.Immunofluorescence and Western Blot results revealed increased expression of HMGB1 and related proteins in the model group,which was reduced in the BG treatment groups.Conclusion:BG exhibited an intervention effect on secondary lung injury following cerebral ischemia-reperfusion,possibly by modulating antioxidant enzyme activity,inhibiting the expression of HMGB1/PI3K/AKT pathway-related proteins,and alleviating secondary lung injury in rats after cerebral ischemia-reperfusion.

关 键 词：黄芩苷 栀子苷 脑缺血再灌注继发肺损伤 肺损伤 HMGB1 PI3K/AKT 

分 类 号：R285.5[医药卫生—中药学]