254 nm连续紫外线与脉冲紫外激光物体表面消毒研究  

Surface disinfection efficacy of 254 nm continuous ultraviolet and pulsed ultraviolet laser

作　　者：赵洁 赵蕾 商怡然 吴金辉 衣颖 邓橙 祁建城 张宗兴 ZHAO Jie;ZHAO Lei;SHANG Yiran;WU Jinhui;YI Ying;DENG Cheng;QI Jiancheng;ZHANG Zongxing(Systems Engineering Institute,Academy of Military Science of the Chinese People’s Liberation Army,National Bio-protection Engineering Center,Tianjin 300161,China)

机构地区：[1]军事科学院系统工程研究院,国家生物防护装备工程技术研究中心,天津300161

出　　处：《中国感染控制杂志》2025年第3期316-322,共7页Chinese Journal of Infection Control

基　　金：国家重点研发计划课题(2021YFC2600304)。

摘　　要：目的对比分析连续紫外线和脉冲紫外激光对病原微生物的杀灭效果。方法采用254 nm紫外汞灯和紫外激光对大肠埃希菌和萎缩芽孢杆菌芽孢染菌玻片进行照射消毒处理,采用载体定量杀灭试验测定在不同辐照剂量下的消毒效果。结果紫外汞灯辐照剂量为245.52 mJ/cm^(2)时,大肠埃希菌和萎缩芽孢杆菌芽孢的杀灭对数值分别为5.00、2.92,平均对数杀灭剂量分别为49.10、84.08 mJ/cm^(2);而紫外激光辐照剂量分别为208.39、206.80 mJ/cm^(2)时,对两种微生物的杀灭对数值分别为6.29、3.32,平均对数杀灭剂量分别为33.13、62.29 mJ/cm^(2)。结论相较于连续紫外线,脉冲紫外激光具有更强的穿透能力,在相同的辐射剂量下对病原微生物的杀灭效果更好,可以对物体表面进行定向消杀。Objective To compare and analyze the germicidal efficacy of continuous ultraviolet and pulsed ultraviolet(UV)lasers on pathogenic microorganisms.Methods Spore slides of Escherichia coli and Bacillus atrophaeus were irradiated using a 254 nm ultraviolet mercury lamp and UV laser.The carrier quantitative germicidal test was conducted to determine the disinfection efficacy at different irradiation doses.Results When the irradiation dose of the ultraviolet mercury lamp was 245.52 mJ/cm^(2),the logarithmic killing values of Escherichia coli and Bacillus atrophaeus spores were 5.00 and 2.92,respectively,and the mean logarithmic killing doses were 49.10 and 84.08 mJ/cm^(2),respectively;When the UV laser irradiation doses were 208.39 and 206.80 mJ/cm^(2),the logarithmic killing values for the two microorganisms were 6.29 and 3.32,respectively,and the mean logarithmic killing doses were 33.13 and 62.29 mJ/cm^(2),respectively.Conclusion Compared with continuous UV radiation,pulsed UV laser has stronger penetration ability,better killing efficacy on pathogenic microorganisms at the same radiation dose,and can conduct targeted disinfection and sterilization on the surface of objects directionally.

关 键 词：紫外线 脉冲紫外激光 连接紫外线 消毒 灭菌 杀灭对数值 

分 类 号：R318.6[医药卫生—生物医学工程]