水稻突变体株系orr2耐盐特性评价及响应盐胁迫关键基因挖掘  

Evaluation of salt tolerance characteristics of rice mutant strain orr2 and mining of key genes responding to salt stress

作　　者：黄思雨 陈思扬 张丹 张帅龙 周璇 刘小林 盛夏冰[2,3] 袁定阳 HUANG Siyu;CHEN Siyang;ZHANG Dan;ZHANG Shuailong;ZHOU Xuan;LIU Xiaolin;SHENG Xiabing;YUAN Dingyang(College of Tropical Agriculture and Forestry,Hainan University,Haikou 570208,China;Hunan Hybrid Rice Research Center,Seed Industry Center of National Technology Innovation Center for Saline-alkali Tolerant Rice,Changsha 410125,China;National Innovation Center for Salt-tolerant Rice Technology in Sanya,Sanya,Hainan 572019,China;College of Agronomy,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]海南大学热带农林学院,海口570208 [2]湖南杂交水稻研究中心,国家耐盐碱水稻技术创新中心种业中心,长沙410125 [3]三亚市国家耐盐碱水稻技术创新中心,海南三亚572019 [4]湖南农业大学农学院,长沙410128

出　　处：《植物生理学报》2025年第2期147-159,共13页Plant Physiology Journal

基　　金：湖南省自然科学基金(2022JJ40296);中央引导地方科技发展资金(2024ZYT002);湖南省“十大技术攻关项目”(2024NK1010);湖南省农业科技创新资金(2024CX08);海南省重点研发专项;海南省重大科技计划(ZDKJ202001)。

摘　　要：盐胁迫是影响水稻正常生产的主要非生物胁迫之一,为解析水稻耐盐机理、挖掘耐盐相关基因,本试验以野生型‘华占’(WT)及其突变体(orr2)为供试材料,对WT与orr2幼苗进行耐盐表型鉴定、生理指标测定及转录组分析。结果表明,与WT相比,orr2存活率、抗氧化酶活性均显著提高,活性氧(ROS)、丙二醛(MDA)含量显著降低。转录组GO富集分析发现,WT和orr2的差异表达基因主要富集在氧化还原途径,从中筛选了4个抗氧化相关基因(OsFe-SOD、OsAPXa、OsGPX4和OsPEX11),并进行了实时荧光定量(qRT-PCR)验证。在4个候选基因启动子上均存在与ORR2的潜在结合位点,推测ORR2有可能结合在候选基因的启动子区域并通过调控其表达水平,从而影响水稻耐盐性,但具体机制还有待进一步验证。研究结果不仅有助于进一步揭示水稻耐盐机理,同时也为水稻耐盐基因挖掘提供了新候选基因。Salt stress is one of the major abiotic stresses affecting the normal production of rice.To analyze the salt tolerance mechanism of rice and excavate salt tolerance-related genes,in this experiment,the wild-type‘Huazhan’(WT)and its mutant strain(orr2)were used as the test materials.Salt stress treatment was carried out during the seedling stage,and the salt tolerance phenotypes,physiological indexes,and transcriptome analysis of WT and orr2 seedlings were conducted. The results showed that compared with WT, the survival rate and antioxidant enzyme activities of orr2 were signiffcantly increased, while the contentsof reactive oxygen species (ROS) and malondialdehyde (MDA) were signiffcantly decreased. Transcriptome GO enrichment analysis revealed that the differentially expressed genes between WT and orr2 were mainly enriched in the redox pathway. Four antioxidant-related genes (OsFe-SOD, OsAPXa, OsGPX4, and OsPEX11) were screened from this and veriffed by real-time ffuorescence quantitative PCR (qRT-PCR). Potential binding sites with ORR2 were present on the promoters of the four candidate genes. It is speculated that ORR2 may bind to the promoter regions of the candidate genes and affect the salt tolerance of rice by regulating their expression levels, but the speciffc mechanism remains to be further veriffed. The research results are not only helpful for further revealing the salt tolerance mechanism of rice, but also provide new candidate genes for the excavation of salt tolerance genes in rice.

关 键 词：水稻 盐胁迫 抗氧化 转录组 

分 类 号：S511[农业科学—作物学]