低温胁迫下不同倍性滇山茶植物激素含量变化及其相关基因的表达分析  

Changes of phytohormone content and expression analysis of related genes in different ploidy Camellia reticulata under low temperature stress

作　　者：高圆圆 雍清青 左杰 屈燕[1] GAO Yuanyuan;YONG Qingqing;ZUO Jie;QU Yan(College of Landscape and Horticulture,Southwest Forestry University,Engineering Technology Research Center of National Forestry and Grassland Administration on Southwest Landscape Architecture,Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization,Kunming 650224,China)

机构地区：[1]西南林业大学园林园艺学院,国家林业和草原局西南风景园林工程技术研究中心,云南省功能性花卉资源及产业化技术工程研究中心,昆明650224

出　　处：《植物生理学报》2025年第2期201-213,共13页Plant Physiology Journal

基　　金：国家“十三五”重点研发计划(2019YFD1001005);云南省“万人计划”青年拔尖人才项目(YNWR-QNBJ-2019-211);云南省教育厅科学研究基金(2025Y0858)。

摘　　要：本研究以二倍体、四倍体、六倍体滇山茶幼苗为实验材料,在低温(–4℃)处理下对其进行表型观测并测定净光合速率(P_(n))、气孔导度(G_(s))、实际光化学速率Y(Ⅱ)和非光化学淬灭(NPQ),以及生长素(IAA)、赤霉素(GA)和脱落酸(ABA)含量并进行转录组分析,对IAA、ABA和GA信号转导相关基因的表达量变化规律及其与IAA、ABA、GA含量之间相关性进行分析。结果表明,随着低温胁迫时间的延长,3种倍性滇山茶生理指标发生较明显的变化,P_(n)、G_(s)、Y(Ⅱ)逐渐下降,NPQ先上升后下降。低温胁迫下的3种倍性滇山茶GA含量呈下降的趋势;ABA含量整体呈上升趋势,但上升下降时间各有差异;IAA呈现不规则变化,二倍体和六倍体滇山茶IAA含量略降,四倍体滇山茶IAA含量相对上升。结合转录组测序对3种倍性滇山茶的IAA、GA和ABA信号转导途径分析,筛选到AUX/IAA、DELLA、PP2C 3个植物激素信号途径相关基因与滇山茶冷胁迫响应显著相关。上述结果为滇山茶耐冷基因克隆及耐冷机制解析提供了理论依据。In this study,diploid,tetraploid and hexaploid Camellia reticulata seedlings were used as experimental materials,and phenotypic observations and measurements of net photosynthetic rate(P_(n)),stomatal conductance(G_(s)),actual photochemical rate Y(II)and non-photochemical quenching(NPQ),as well as growth hormone(IAA),gibberellin(GA)and abscisic acid(ABA)content and transcriptome analysis were performed to analyze the expression pattern changes of IAA,ABA and GA signal transduction-related genes and their correlation with IAA,ABA and GA content.The results showed that with the prolongation of low temperature stress, the physiological indicators of three ploidy C. reticulata changed more obviously, with the gradual decrease of P_(n), G_(s) and Y(II), and the ffrst increase and then decrease of NPQ. Under low temperature stress, the GA content of three ploidy of C. reticulata showed a decreasing trend. The ABA content showed an overall increasing trend, but the time of increase and decrease varied. And the IAA content showed irregular changes, with a slight decrease in the IAA content of the diploid and hexaploid haplotypes, and a relative increase in the IAA content of the tetraploid haplotypes of C. reticulata. Combined with the transcriptome sequencing analysis of IAA, GA and ABA signaling pathways in three ploidy of C. reticulata, three phytohormone signaling pathways related genes, namely AUX/IAA, DELLA and PP2C, were identiffed as signiffcantlyassociated with the response to cold stress in C. reticulata. These results provide a theoretical basis for the cloning of cold tolerance genes and the analysis of cold tolerance mechanism in C. reticulata.

关 键 词：滇山茶 低温 植物激素 差异表达基因 信号转导 

分 类 号：S685.14[农业科学—观赏园艺]