2021-2023年陕西省西安市非伤寒沙门菌分子分型和耐药基因携带情况分析  

Molecular typing and drug resistance gene carriage of non-typhoidal Salmonella in Xi'an,Shaanxi,2021−2023

作　　者：李浩[1] 栾阳[1] 夏茹意 赵贺 Li Hao;Luan Yang;Xia Ruyi;Zhao He(Microbiology and Biomedical Laboratory Department,Xi'an Municipal Center for Disease Control and Prevention,Xi'an 710054,Shaanxi,China;Department of Epidemiology and Biostatistics,School of Public Health,Xi'an Jiaotong University Health Science Center,Xi'an 710041,Shaanxi,China)

机构地区：[1]西安市疾病预防控制中心微生物与生物检验科,陕西西安710054 [2]西安交通大学医学部公共卫生学院流行病与统计学系,陕西西安710041

出　　处：《疾病监测》2025年第1期122-126,共5页Disease Surveillance

基　　金：陕西省自然科学基础研究项目(No.2022JQ-768);西安市卫生健康委员会科研项目(No.2024yb26)。

摘　　要：目的分析2021—2023年陕西省西安市非伤寒沙门菌的分子分型和耐药基因携带情况,了解其种群结构和耐药基因分布,为制定针对性的沙门菌防控策略提供科学依据。方法利用MGISEQ-200测序平台对非伤寒沙门菌进行全基因组测序,拼接完成后利用EntroBase提供的核心基因组多位点序列分型(cgMLST)的方案构建全基因组等位基因最小生成树,利用ResFinder 4.2.0软件进行耐药基因鉴定。结果96株非伤寒沙门菌共分为17个HCC50型别,其中HCC50_1~HCC50_4为主要流行型别。9株HCC50_3型沙门菌均为2021年所分离,等位基因差异数均<10个,参考流行病学资料,可能有共同来源。HCC50_1型别内部,2个单克隆子型别HCC5_1和HCC5_2在2022和2023年均有检出,显示这2个单克隆株在本地流行。HCC50_2和HCC50_4可以分为25个HCC5型别,呈散发。耐药基因方面,96株非伤寒沙门菌β-内酰胺类耐药基因bla TEM-1、氨基糖苷类耐药基因aph(3'')-Ib和aph(6)-Id总携带率均为80.21%(77/96),四环素类耐药基因tet(A)、tet(B)和tet(M)总携带率为69.79%(67/96),磺胺类抗生素耐药基因sul2基因总携带率为73.96%(71/96)。结论全基因组cgMLST分型分辨率高,可以多层次地描述沙门菌种群结构,鉴定散在暴发克隆,是流行病学溯源的重要工具。西安市非伤寒沙门菌存在优势流行克隆群,且普遍携带多种耐药基因,表现出多重耐药趋势,应加强耐药监测和管理。Objective To analyze the molecular typing and drug resistance gene carriage of non-typhoidal Salmonella in Xi'an,Shaanxi province,from 2021 to 2023,explore its population structure and distribution of drug resistance genes,and provide evidence for the development of targeted Salmonella infection prevention and control strategies.Methods The whole genome of 96 non-typhoid Salmonella strains was sequenced by using the MGISEQ-200 sequencing platform.After the assembly was completed,the minimum spanning tree of the whole genome alleles was constructed based on the core genome multilocus sequence typing(cgMLST)scheme provided by EntroBase,and software ResFinder 4.2.0 was used to identify drug-resistant genes.Results The 96 strains of non-typhoidal Salmonella were divided into 17 HCC50 types,with HCC50_1 to HCC50_4 being the main prevalent types.The 9 strains of Salmonella HCC50_3 were all isolated in 2021,and the number of allele differences was less than 10.According to epidemiological data,they might have a common source.In HCC50_1 type,two single clone subtypes,HCC5_1 and HCC5_2,were detected in both 2022 and 2023,indicating the spread of two monoclonal strains in Xi'an.HCC50_2 and HCC50_4 could be further divided into 25 HCC5 subtypes,exhibiting sporadic distribution.β-lactam resistant gene blatEM-1 was carried by 80.21%(77/96)of the 96 non-typhoidal Salmonella isolates,and aminoglycoside resistant genes aph(3)-Ib and aph(6)-Id were also carried by 80.21%(77/96)of the isolates,tetracycline resistant genes tet(A),tet(B),and tet(M)were carried by 69.79%(67/96)of the isolates,and sulfonamide resistant gene sul2 was carried by 73.96%(71/96)of the isolates.Conclusion Whole genome cgMLST typing has high resolution to describe the population structure of Salmonella at multiple levels and identify sporadic outbreak clones.It is an important tool for epidemiological tracing.Predominant epidemic clones of non-typhoidal Salmonella exist in Xi'an,and most of them carry multiple drug-resistant genes,showing a trend of multidr

关 键 词：非伤寒沙门菌 分子分型 耐药基因 

分 类 号：R211[医药卫生—中医学] R373