16S rRNA基因测序下探索腺样体局部微生物菌群  

Exploring local microbial communities in adenoids through 16S rRNA gene sequencing

作　　者：袁洛花 刘海兵 李文丽 彭中华 马玉玲 邹剑[1] YUAN Luohua;LIU Haibing;LI Wenli;PENG Zhonghua;MA Yuling;ZOU Jian(Department of Otolaryngology Head and Neck Surgery,West China Hospital,Sichuan University,Chengdu,610000,China;Otolaryngology Department of Sichuan Maternal and Child Health Hospital)

机构地区：[1]四川大学华西医院耳鼻咽喉头颈外科,成都610000 [2]四川省妇幼保健院耳鼻喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2025年第1期51-56,共6页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:通过分析腺样体局部微生物菌群,探讨“病原体储存库”假说。方法:在70°鼻内镜引导下,用无菌拭子采集受试者腺样体隐窝内分泌物,送至实验室提取样本DNA后进行标准细菌16S全长测序分析。结果:在物种水平上测得腺样体隐窝内排前3位的微生物菌群是多杀巴氏杆菌(18.78%),具核梭杆菌(11.42%),肺炎链球菌(9.38%)。结论:腺样体局部微生物菌群具有高度的多样性,包含了来自口腔及消化道的微生物菌群,我们的研究结果也支持了腺样体是“病原体储存库”这一假说。Objective:To explore the hypothesis of"pathogen storage pool"by analyzing the local microbial community of adenoids.Methods:Under the guidance of a 70°nasal endoscope,sterile swabs were used to collect secretions from the adenoid crypts of the subjects.The samples were sent to the laboratory for DNA extraction and standard bacterial 16S full-length sequencing analysis.Results:At the species level,the top three microbial communities in adenoid crypts were Bacillus subtilis(18.78%),Fusobacterium pyogenes(11.42%),and Streptococcus pneumoniae(9.38%).Conclusion:The local microbial community of adenoids exhibits a high degree of diversity,including microbial communities from the oral cavity and gastrointestinal tract.Our research results support the hypothesis that adenoids act as a"pathogen reservoir".

关 键 词：腺样体 微生物菌群 16S rRNA 

分 类 号：R766.16[医药卫生—耳鼻咽喉科]