妊娠期高血压疾病患者血清lncRNA OIP5-AS1和miR-374a-5p表达对下肢深静脉血栓的预测价值分析  

The predictive value of serum lncRNA OIP5⁃AS1 and miR⁃374a⁃5p expression of lower limb deep vein thrombosis in patients with hypertensive disorder complicating pregnancy

作　　者：于运萍 李婷[1] 王诗翔 杨小芳 Yu Yunping;Li Ting;Wang Shixiang;Yang Xiaofang(First Department of Obstetrics,Handan Central Hospital,Handan Hebei 056000,P.R.China)

机构地区：[1]邯郸市中心医院产一科,河北邯郸056000

出　　处：《中国计划生育和妇产科》2025年第1期57-61,83,共6页Chinese Journal of Family Planning & Gynecotokology

基　　金：河北省医学科学研究课题计划项目(项目编号:20220477)。

摘　　要：目的探讨妊娠期高血压疾病(hypertensive disorder complicating pregnancy,HDCP)患者血清长链非编码RNA Opa相互作用蛋白5-反义转录物1(lncRNA OIP5-AS1)和微小RNA-374a-5p(miR-374a-5p)表达与下肢深静脉血栓(deep vein thrombosis,DVT)发生的关系及其预测价值。方法选取邯郸市中心医院2022年5月至2024年1月收治的149例HDCP孕妇(HDCP组)和144例健康孕妇(对照组)作为研究对象。将HDCP组根据是否并发下肢DVT分为DVT组(44例)和非DVT组(105例)。收集患者临床资料并检测凝血指标;荧光定量PCR法检测血清lncRNA OIP5-AS1和miR-374a-5p表达水平;ENCORI网站分析lncRNA OIP5-AS1与miR-374a-5p的核酸序列互补位点。比较对照组和HDCP组、非DVT组和DVT组临床资料、凝血指标、血清lncRNA OIP5-AS1和miR-374a-5p表达水平;分析HDCP并发下肢DVT患者血清lncRNA OIP5-AS1、miR-374a-5p与凝血指标的相关性,探讨影响HDCP患者发生下肢DVT的因素及血清lncRNA OIP5-AS1和miR-374a-5p对HDCP患者并发下肢DVT的预测效能。结果与对照组比较,HDCP组血小板计数(PLT)、纤维蛋白原(FIB)、D-二聚体(D-D)、血清lncRNA OIP5-AS1、miR-374a-5p表达水平显著升高,凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)显著降低(P<0.05)。与非DVT组比较,DVT组PLT、FIB、D-D、血清lncRNA OIP5-AS1、miR-374a-5p表达水平显著升高,TT、PT、APTT显著降低(P<0.05)。生物信息学分析显示,lncRNA OIP5-AS1与miR-374a-5p核酸序列存在互补位点。HDCP并发下肢DVT患者血清lncRNA OIP5-AS1与miR-374a-5p呈正相关(P<0.05);血清lncRNA OIP5-AS1和miR-374a-5p均与PLT、FIB、D-D呈正相关,与TT、PT、APTT呈负相关(P<0.05)。PLT、FIB、D-D、lncRNA OIP5-AS1、miR-374a-5p是影响HDCP患者并发下肢DVT的独立危险因素,TT、PT、APTT是独立保护因素(P<0.05)。血清lncRNA OIP5-AS1、miR-374a-5p两者联合预测HDCP患者发生下肢DVT的曲线下面积(AUC)(0.943)大于两者单独预测的AUC(0.739、0.8Objective To investigate the relationship between the expression of serum long non-coding RNA Opa interacting protein 5-antisense transcript 1(lncRNA OIP5-AS1)and microRNA-374a-5p(miR-374a-5p)and the occurrence of lower limb deep vein thrombosis(DVT)in patients with hypertensive disorder complicating pregnancy(HDCP)and its predictive value.Methods 149 pregnant women with HDCP(HDCP group)and 144 healthy pregnant women(control group)admitted to Handan Central Hospital from May 2022 to January 2024 were selected as the research subjects.The HDCP group were divided into DVT group(44 cases)and non-DVT group(105 cases)according to whether there were complications of lower limb DVT.Clinical data of patients were collected and coagulation indexes were detected;the expression levels of serum lncRNA OIP5-AS1 and miR-374a-5p were detected by fluorescence quantitative PCR.ENCORI website analyzed the nucleic acid sequence complementary sites of lncRNA OIP5-AS1 and miR-374a-5p.The clinical data,coagulation indexes,serum IncRNA OIP5-AS1 and miR-374a-5p expression levels of control group and HDCP group,non-DVT group and DVT group were compared.The correlation between serum IncRNA OIP5-AS1 and miR-374a-5p and coagulation indexes in patients with HDCP complicated with lower limb DVT were analyzed.The factors affecting the occurrence of lower limb DVT in patients with HDCP and the predictive power of serum lncRNA OIP5-AS1 and miR-374a-5p on the occurrence of lower limb DVT in HDCP patients were explored.Results Compared with control group,the platelet count(PLT),fibrinogen(FIB),D-dimer(D-D),serum IncRNA OIP5-AS1 and miR-374a-5p expression levels in HDCP group were significantly increased,thrombin time(TT),prothrombin time(PT)and activated partial thromboplastin time(APTT)were significantly decreased(P<0.05).Compared with non-DVT group,the PLT,FIB,D-D and serum lncRNA OIP5-AS1 and miR-374a-5p expression levels of in DVT group were significantly increased,while TT,PT and APTT were significantly decreased(P<0.05).Bioinformatics analys

关 键 词：妊娠期高血压疾病 长链非编码RNA Opa相互作用蛋白5-反义转录物1 微小RNA-374a-5p 凝血指标 下肢深静脉血栓 

分 类 号：R714.246[医药卫生—妇产科学]