Ghrelin及Gas6基因多态性与冠心病患者发生风险的关系分析  

Relationship between Ghrelin and Gas6 gene polymorphisms and risk of coronary heart disease

作　　者：洪必莹 杨露 钟锐 周远林[1] HONG Biying;YANG Lu;ZHONG Rui;ZHOU Yuanlin(Cardiovascular Department,Chengdu Second People's Hospital,Chengdu 610000,China)

机构地区：[1]成都市第二人民医院心血管内科,四川成都610000

出　　处：《西部医学》2025年第3期393-397,402,共6页Medical Journal of West China

基　　金：四川省医学青年创新科研课题计划(Q20064)。

摘　　要：目的探讨饥饿激素(Ghrelin)及生长抑制子G蛋白耦联受体激酶激活剂6(Gas6)基因多态性与冠心病患者发生风险的关系分析。方法选取2022年5月—2023年5月本院收治的冠心病患者100例作为观察组,另选取100例健康体检者作为对照组,酶联免疫吸附法测定血清Ghrelin及Gas6水平,采用TaqMan-PCR法测定Ghrelin及Gas6基因多态性,并分析Ghrelin及Gas6基因多态性与冠心病患者发生风险的关系。结果与对照组相比,观察组血清Ghrelin水平明显降低,Gas6水平明显升高(P<0.05);对照组Ghrelin基因AA、CA、CC基因型频率分别为47.00%、36.00%、17.00%,观察组分别为11.00%、18.00%、71.00%,对照组C、A等位基因频率分别为35.00%、65.00%,观察组分别为80.00%、20.00%,组间具有统计学差异(P<0.05);与对照组相比,观察组Ghrelin水平在CC基因型中降低(P<0.05),在AA、CA基因型中无明显差异(P>0.05);用Ghrelin基因座的3种不同基因型作为独立变量,以各基因座的野生型纯合子为基准,其他基因型为虚拟变量,拟合二元Logistic回归模型,结果显示,Ghrelin基因CC基因型升高了冠心病的发生风险(OR=2.967),组间具有统计学差异(P<0.05)。对照组Gas6基因GG、GA、AA基因型频率分别为40.00%、36.00%、24.00%,观察组分别为52.00%、44.00%、4.00%,对照组A、G等位基因频率分别为42.00%、58.00%,观察组分别为26.00%、74.00%,组间具有统计学差异(P<0.05)。与对照组相比,观察组Gas6水平在GA基因型中升高,在AA基因型中降低(P<0.05),在GG基因型中无明显差异(P>0.05);用Gas6基因座的3种不同基因型作为独立变量,以各基因座的野生型纯合子为基准,其他基因型为虚拟变量,拟合二元Logistic回归模型,结果显示,Gas6基因GA基因型升高了冠心病的发生风险(OR=2.157),AA基因型降低了冠心病的发生风险(OR=0.523),组间具有统计学差异(P<0.05)。结论Ghrelin及Gas6水平与冠心病具有密切关系,Ghrelin及Gas6基因多态性�Objective To investigate the relationship between Ghrelin and Gas6 gene polymorphisms and the risk of coronary heart disease.Methods From May 2022 to May 2023,100 patients with coronary heart disease admitted to our hospital were selected as the study subjects and recorded as the observation group,and another 100 healthy subjects were selected as the control group.Serum Ghrelin and Gas6 levels were determined by enzyme-linked immunosorbent assay.Ghrelin and Gas6 gene polymorphisms were determined by TaqMan-PCR,and the relationship between Ghrelin and Gas6 gene polymorphisms and the risk of coronary heart disease was analyzed.Results Compared with control group,the serum Ghrelin level in observation group was significantly decreased,Gas6 level was significantly increased(P<0.05);Genotype frequencies of AA,CA and CC were 47.00%,36.00% and 17.00% in control group,11.00%,18.00% and 71.00% in observation group,allele frequencies of C and A were 35.00% and 65.00% in control group,and 80.00% and 20.00% in observation group,respectively.There was statistical significance between groups(P<0.05).Compared with control group,Ghrelin level in CC genotype was decreased(P<0.05),but there was no significant difference in AA and CA genotype in observation group(P>0.05).Three different genotypes of Ghrelin loci were used as independent variables,wild-type homozygotes of each loci were used as reference,and other genotypes were used as dummy variables.Binary Logistic regression model was fitted,and the results showed that CC genotype of Ghrelin gene increased the risk of coronary heart disease(OR=2.967).There was statistical significance between groups(P<0.05).The frequencies of GG,GA and AA genotypes were 40.00%,36.00% and 24.00% in the control group,52.00%,44.00% and 4.00% in the observation group,and the frequencies of A and G alleles were 42.00% and 58.00% in the control group,26.00% and 74.00% in the observation group.There was statistical significance between groups(P<0.05).Compared with the control group,Gas6 level in the ob

关 键 词：饥饿激素 生长抑制子G蛋白耦联受体激酶激活剂6 基因多态性 冠心病 发生风险 

分 类 号：R541.4[医药卫生—心血管疾病]