重度痤疮患者皮肤菌群多样性研究  

Study of skin microbiota diversity in patients with severe acne

作　　者：黄舒燕 黄卫东 蔡蕾 黄剑清 雷小英 郭宏恩 张雪梅 HUAN-Shuyan;HUAN-Weidong;CAI Lei;HUAN-Jianqing;LEI Xiaoying;GUO Hongen;ZHAN-Xuemei(Departmentof Dermatology,Dermatology Hospitalof Fuzhou,Fuzhou,Fujian 350025,China)

机构地区：[1]福建省福州市第一总医院皮肤病防治院皮肤科,福州350025

出　　处：《福建医药杂志》2025年第2期5-8,F0002,共5页Fujian Medical Journal

基　　金：福建省卫生健康科技计划项目(2021QNA065)。

摘　　要：目的探讨重度痤疮患者皮损内微生物菌群的多样性。方法选择福州市第一总医院皮肤病防治院皮肤科接诊的30例重度痤疮患者作为观察组,20例正常健康人作为对照组,利用细菌16S核糖体DNA(16S rDNA)和真菌内转录间隔区1(Internal Transcribed Spacer 1,ITS1)扩增子测序技术,检测痤疮患者皮损内及正常人面部皮肤的细菌和真菌群落结构,分析两组的物种多样性。结果观察组的细菌ace指数、chao指数高于对照组,差异有统计学意义(P<0.001),表明细菌总量明显增多;同时,与对照组相比,shannon指数降低,差异有统计学意义(P<0.001),simpson指数升高,差异有统计学意义(P<0.001),且β多样性分析差异有统计学意义(P=0.001),提示痤疮皮损内细菌物种的集中度高、多样性较低,这与丙酸杆菌属显著性上升有关,而且痤疮丙酸杆菌明显升高。观察组的真菌ace指数和chao指数上升,差异有统计学意义(P<0.001),这主要与马拉色菌属丰度上升有关。结论通过对比重度痤疮患者与正常对照组的皮肤菌群,发现两组在菌群的组成、丰富度和多样性上差异有统计学意义,为深入了解重度痤疮的发病机制提供参考,也为重度痤疮的治疗提供了新的研究方向。Objective To investigate the skin microbiota species diversity in patients with severe acne.Methods Thirty patients with severe acne as the observation group and 20 healthy individuals as the control group were collected from Department of Dermatology,Dermatology Hospital of Fuzhou.The bacterial 16S ribosomal DNA(16S rDNA)and fungal internal transcribed spacer 1(ITS1)amplicon sequencing technology was utilized to detect the bacterial and fungal community structures within the lesions of acne patients and on the facial skin of healthy individuals.The species diversity of the two groups was analyzed.Results The bacterial ace index and chao index of the observation group were higher than those of the control group,and the difference was statistically significant(P<0.001).It indicated that the total amount of bacteria increased significantly.Compared with the control group,the shannon index of the observation group decreased,and the difference was statistically significant(P<0.001),simpson index increased and the difference was statistically significant(P<0.001).There was a statistically significant difference byβdiversity analysis(P=0.001).It indicated that the concentration of bacterial species in acne lesions was high and the diversity was low,which was related to the significant increase of Propionibacterium and Propionibacterium acnes.The ace index and chao index of fungi in the observation group increased,and the difference was statistically significant(P<0.001),which was mainly related to the increase of Malassezia abundance.Conclusion By comparing the skin flora of severe acne patients and normal healthy individuals,it was found that there were statistically significant differences in the composition,richness and diversity of the flora between the two groups,which provided a reference for in-depth understanding of the pathogenesis of severe acne,and also provided a new research direction for the treatment of severe acne.

关 键 词：重度痤疮 细菌 真菌 多样性 

分 类 号：R758.73[医药卫生—皮肤病学与性病学]