银杏叶提取物对COPD肺泡巨噬细胞自噬相关蛋白的影响  

Effects of Ginkgo biloba extract on autophagy related proteins in COPD alveolar macrophages

作　　者：苏醒 齐红松 陈高瑛 SU Xing;QI Hongsong;CHEN Gaoying(Department of Respiratory and Critical Care Medicine,TCM Ward,the First Affiliated Hospital of Henan University,Kaifeng 475000,China)

机构地区：[1]河南大学第一附属医院呼吸与危重症医学科中医联合病区,开封475000

出　　处：《华夏医学》2025年第1期76-81,共6页Acta Medicinae Sinica

基　　金：2022年度河南省医学科技攻关计划项目(LHGJ20220648)。

摘　　要：目的观察银杏叶提取物(GBE)对慢性阻塞性肺疾病(COPD)肺泡巨噬细胞自噬相关蛋白的影响。方法选取河南大学第一附属医院2021年6月至2023年10月收治的100例COPD患者,随机分为常规组和GBE组,每组50例。常规组予以常规药物治疗,GBE组采用GBE辅助常规药物治疗,所选GBE为舒血宁注射液,比较两组患者的炎症指标、氧化应激指标、肺泡巨噬细胞自噬相关蛋白及肺功能指标。结果在不同治疗方案下,GBE组的TNF-α、IL-8、IL-10、IFN-γ水平均低于常规组,差异有统计学意义(P<0.05);GBE组的SOD、GSH水平均高于常规组,LDH、NO水平均低于常规组(P<0.05);GBE组的LC3蛋白、PI3K蛋白、Akt蛋白、mTOR蛋白的相对表达量均低于常规组(P<0.05);GBE组的FEV_(1)、FVC、PEF、MVV测得值均高于常规组(P<0.05)。结论银杏叶提取物辅助治疗COPD能有效减轻气道炎症反应及氧化应激损伤,并促进患者肺功能恢复,其作用机制可能与抑制巨噬细胞PI3K/Akt/mTOR通路相关。Objective To observe of the effect of Ginkgo biloba extract(GBE)on autophagy related proteins in alveolar macrophages chronic obstructive pulmonary disease(COPD).Methods 100 COPD patients admitted to the First Affiliated Hospital of Henan University from June 2021 to October 2023 were randomly divided into group and GBE group,with 50 patients in each group.The conventional group were received conventional drug treatment,while the GBE group were received GBE assisted conventional drug treatment.The selected GBE was Shuxuening injection.The inflammatory indicatorsoxidative stress indicators,autophagy related proteins,and lung function indicators were compared between the two groups of patients.Results Under different treatment regimens,the levels of TNF-α,IL-8,IL-10,and IFN-γin the GBE group were lower than those in the conventional group,showing a statistically significant difference(P<0.05).The SOD and GSH levels in the GBE group were higher than those in the control group,while LDH and NO levels were lower than those in the control group(P<0.05).The relative expression levels of LC3,PI3K,Akt,and mTOR in the GBE group were lower than those in the conventional group(P<0.05).The FEV_(1),FVC,PEF,and MVV the GBE group were higher than those the conventional group(P<0.05).Conclusion Ginkgo biloba extract as an adjuvant therapy for COPD reduce airway inflammation and oxidative stress damage,and promote the recovery of lung function in patientsmechanism be related to the inhibition of PI3K/Akt/mTOR pathway.

关 键 词：慢性阻塞性肺疾病 银杏叶提取物 巨噬细胞 PI3K/Akt/mTOR信号通路 

分 类 号：R56[医药卫生—呼吸系统]